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Characterization of Decorin of the Human Yellow Ligament
http://hdl.handle.net/10129/70
http://hdl.handle.net/10129/7035d802b8-db49-4138-a2e4-58e99f93b053
名前 / ファイル | ライセンス | アクション |
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KJ00004190598.pdf (673.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-12-07 | |||||
タイトル | ||||||
タイトル | Characterization of Decorin of the Human Yellow Ligament | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | decorin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glycosaminoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | yellow ligament | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dermatan sulfate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | デコリン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | プロテオグリカン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | グリコサミノグリカン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 黄色靱帯 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | デルマタン硫酸 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Takeuchi, Kazunari
× Takeuchi, Kazunari× Harata, Seiko× Takagaki, Keiichi× Endo, Masahiko |
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著者(ヨミ) | ||||||
姓名 | タケウチ, カズナリ | |||||
著者(ヨミ) | ||||||
姓名 | ハラタ, セイコウ | |||||
著者(ヨミ) | ||||||
姓名 | タカガキ, ケイイチ | |||||
著者(ヨミ) | ||||||
姓名 | エンドウ, マサヒコ | |||||
著者別名 | ||||||
姓名 | 竹内, 和成 | |||||
著者別名 | ||||||
姓名 | 原田, 征行 | |||||
著者別名 | ||||||
姓名 | 高垣, 啓一 | |||||
著者別名 | ||||||
姓名 | 遠藤, 正彦 | |||||
著者所属 | ||||||
弘前大学医学部整形外科学講座 | ||||||
著者所属 | ||||||
弘前大学医学部整形外科学講座 | ||||||
著者所属 | ||||||
弘前大学医学部第一生化学講座 | ||||||
著者所属 | ||||||
弘前大学医学部第一生化学講座 | ||||||
著者所属 | ||||||
Department of Orthopaedic Surgery, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Orthopaedic Surgery, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
First Department of Biochemistry, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
First Department of Biochemistry, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Orthopaedic Surgery, Hirosaki University School of Medicine | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Decorin, a component of the low-molecular-weight proteoglycans (PG) of yellow ligaments was purified, and the structures of the core protein and the glycosaminoglycan (GAG) chains were analyzed. The crude PG fraction extracted under dissociative conditions was subjected to DEAE-Sephacel ion-exchange chromatography, gel-filtration on Sepharose CL-4B, and Octyl-Sepharose CL-4B chromatography, and decorin was obtained. The amino-terminal amino acid sequence of decorin was found to be identical to those of human bone PG-II and human fetal membrane PG-II. The analyses of the GAG chains using two-dimensional electrophoresis on cellulose acetate membranes, affinity high-performance liquid chromatography (HPLC) with a hydroxyapatite column and gel-filtration HPLC after chondroitinase digestion, showed the presence of dermatan sulfate (DS) chains and chondroitin sulfate (CS). Further, it was suggested that CS located in the reducing termini and DS in the non-reducing termini in the hybrid chain. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 弘前医学. 56(2/3/4), 2005, 45-54 | |||||
書誌情報 |
弘前医学 巻 56, 号 2/3/4, p. 45-54, 発行日 2005-03-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1721 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 490 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト黄色靱帯のデコリンの性状 | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | ヒト オウショク ジンタイ ノ デコリン ノ セイジョウ | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 基礎医学 | |||||
出版者 | ||||||
出版者 | 弘前大学医学部・弘前医学会 | |||||
資源タイプ | ||||||
値 | Article |