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Constituent Proteoglycans of the Human Yellow Ligament Extracellular Matrix
http://hdl.handle.net/10129/196
http://hdl.handle.net/10129/1963bf23ad3-7612-476e-9e77-36d3d2477a30
名前 / ファイル | ライセンス | アクション |
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KJ00004529552.pdf (847.2 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-05-02 | |||||
タイトル | ||||||
タイトル | Constituent Proteoglycans of the Human Yellow Ligament Extracellular Matrix | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ヒト黄色靭帯 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 細胞外マトリックス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | プロテオグリカン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | デコリン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | バイグリカン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human yellow ligament | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteoglycan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | extracellular matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | decorin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biglycan | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
成田, 穂積
× 成田, 穂積× 武田, 裕介× 原田, 征行× 高垣, 啓一× 遠藤, 正彦 |
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著者(ヨミ) | ||||||
姓名 | ナリタ, ホズミ | |||||
著者(ヨミ) | ||||||
姓名 | タケダ, ユウスケ | |||||
著者(ヨミ) | ||||||
姓名 | ハラタ, セイコウ | |||||
著者(ヨミ) | ||||||
姓名 | タカガキ, ケイイチ | |||||
著者(ヨミ) | ||||||
姓名 | エンドウ, マサヒコ | |||||
著者別名 | ||||||
姓名 | Narita, Hozumi | |||||
著者別名 | ||||||
姓名 | Takeda, Yusuke | |||||
著者別名 | ||||||
姓名 | Harata, Seiko | |||||
著者別名 | ||||||
姓名 | Takagaki, Keiichi | |||||
著者別名 | ||||||
姓名 | Endo, Masahiko | |||||
著者所属 | ||||||
Department of Biochemistry, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Orthopaedic Surgery, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Orthopaedic Surgery, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Biochemistry, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
Department of Biochemistry, Hirosaki University School of Medicine | ||||||
著者所属 | ||||||
弘前大学医学部第一生化学教室 | ||||||
著者所属 | ||||||
弘前大学医学部整形外科学教室 | ||||||
著者所属 | ||||||
弘前大学医学部整形外科学教室 | ||||||
著者所属 | ||||||
弘前大学医学部第一生化学教室 | ||||||
著者所属 | ||||||
弘前大学医学部第一生化学教室 | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Three types of proteoglycans (PG-I, II and III) present in the human yellow ligament extracellular matrix were separated using a combination of CsCl isopycnic density gradient centrifugation, DEAE-Sephacel ion-exchange chromatography, gel-filtration on Sepharose CL-4B and CL-2B, and Octyl-Sepharose CL-4B chromatography. Proteoglycan-I was a high-molecular-weight proteoglycan, which was excluded by Sepharose CL-4B, and its molecular weight, estimated using sodium dedecyl sulfate-polyacrylamide gel electrophoresis, was over 350,000. The glycosaminoglycan chain of PG-I was composed of chondroitin 6-sulfate, and PG-I appeared to be an aggrecan-type proteoglycan. Proteoglycan-II and III were low-molecular-weight proteoglycans with molecular weights of 190,000 and 105,000, respectively. The core protein sizes of PG-II and III were similar, 44,000, but anti-human decorin antibody reacted with PG-III, not with PG-II. The glycosaminoglycan chain of PG-II contained dermatan sulfate and chondroitin 6-sulfate, whereas that of PG-III contained the former only. In the light of these results, PG-II and III were suggested to be biglycan- and decorin-type proteoglycans, respectively. The major proteoglycan in the human yellow ligament extracellular matrix was PG-III. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 弘前医学. 57(2/3/4), 2006, p.49-58 | |||||
書誌情報 |
弘前医学 巻 57, 号 2/3/4, p. 49-58, 発行日 2006-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1721 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 491.1 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト黄色靭帯の細胞外マトリックスを構成するプロテオグリカン | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | ヒト オウショク ジンタイ ノ サイボウガイ マトリックス オ コウセイスル プロテオグリカン | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 基礎医学 | |||||
出版者 | ||||||
出版者 | 弘前大学医学部・弘前医学会 | |||||
資源タイプ | ||||||
値 | Article |