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TGF-β is a growth factor that has pleiotropic effects on\na wide range of target cells and induces differentiation of basal keratinocytes. Therefore. TGF-β is postulated to\ninhibit BPAG1 expression. However. previous report conversely demonstrated an increase of BPAG1 expression\nby TGF-β. In this study. to understand regulatory role of TGF-β on BPAG1 functions. we examined the effect\nof TGF-β on BPAG1 gene expression using cultured keratinocytes. This study showed that BPAG1 mRNA\nexpression was inhibited by TGF-β1 in concentration higher than 1.0 ng/ml. Furthermore. incubation of the\ncells with TGF-β1 in the presence of cycloheximide demonstrated that newly synthesized protein was required\nfor BPAG1 regulation. To understand the detailed mechanisms of BPAGI modulation by TGF-β, we preformed\ntransient transfection assay with a BPAGI promoter-CAT construct to know the detailed mechanisms of BPAG1\nmodulation by TGF-β. 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Inhibitory Effect of TGF-β on Expression of 230KD Bullous Pemphigoid Antigen
http://hdl.handle.net/10129/689
http://hdl.handle.net/10129/6896c151c86-5178-4065-b544-4eebd9125c6a
名前 / ファイル | ライセンス | アクション |
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AN00211444_59_7.pdf (432.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-07-27 | |||||
タイトル | ||||||
タイトル | Inhibitory Effect of TGF-β on Expression of 230KD Bullous Pemphigoid Antigen | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Keratinocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BPAG1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TGF-β | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IFN-γ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 表皮細胞 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Harada, Ken
× Harada, Ken× Tamai, Katsuto× Nakano, Hajime× Sasaki, Chiaki× Hanada, Katsumi× Hashimoto, Isao× Sawamura, Daisuke |
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著者(ヨミ) | ||||||
姓名 | ハラダ, ケン | |||||
著者(ヨミ) | ||||||
姓名 | タマイ, カツト | |||||
著者(ヨミ) | ||||||
姓名 | ナカノ, ハジメ | |||||
著者(ヨミ) | ||||||
姓名 | ササキ, チアキ | |||||
著者(ヨミ) | ||||||
姓名 | ハナダ, カツミ | |||||
著者(ヨミ) | ||||||
姓名 | ハシモト, イサオ | |||||
著者(ヨミ) | ||||||
姓名 | サワムラ, ダイスケ | |||||
著者別名 | ||||||
姓名 | 原田, 研 | |||||
著者別名 | ||||||
姓名 | 玉井, 克人 | |||||
著者別名 | ||||||
姓名 | 中野, 創 | |||||
著者別名 | ||||||
姓名 | 佐々木, 千秋 | |||||
著者別名 | ||||||
姓名 | 花田, 勝美 | |||||
著者別名 | ||||||
姓名 | 橋本, 功 | |||||
著者別名 | ||||||
姓名 | 澤村, 大輔 | |||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
Dermatology Section. Aomori Prefectural Central Hospital | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
著者所属 | ||||||
青森県立中央病院皮膚科 | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 230-kD bullous pemphigoid antigen (BPAG1) is known as an autoantigen in bullous pemphigoid and is expressed exclusively in proliferating basal keratinocytes. TGF-β is a growth factor that has pleiotropic effects on a wide range of target cells and induces differentiation of basal keratinocytes. Therefore. TGF-β is postulated to inhibit BPAG1 expression. However. previous report conversely demonstrated an increase of BPAG1 expression by TGF-β. In this study. to understand regulatory role of TGF-β on BPAG1 functions. we examined the effect of TGF-β on BPAG1 gene expression using cultured keratinocytes. This study showed that BPAG1 mRNA expression was inhibited by TGF-β1 in concentration higher than 1.0 ng/ml. Furthermore. incubation of the cells with TGF-β1 in the presence of cycloheximide demonstrated that newly synthesized protein was required for BPAG1 regulation. To understand the detailed mechanisms of BPAGI modulation by TGF-β, we preformed transient transfection assay with a BPAGI promoter-CAT construct to know the detailed mechanisms of BPAG1 modulation by TGF-β. The results revealed that calcium and IFN-γ inhibited BPAG1 expression at transcriptional level, but TGF-β1 is not responsible for that transcriptional inhibition, suggesting that TGF-β may have differential molecular mechanism for down-regulation of BPAG1 gene expression from the events induced by IFN-γ. |
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引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 弘前医学. 59, 2007, p.7-14 | |||||
書誌情報 |
弘前医学 巻 59, 号 1, p. 7-14, 発行日 2007-11-15 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1713 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 494.8 | |||||
その他のタイトル | ||||||
その他のタイトル | 230-kD類天疱瘡抗原発現に対するTGF-βの抑制効果 | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | 230-kDルイ テンホウソウ コウゲン ハッソウ ニ タイスル TGF-β ノ ヨクセイ コウカ | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 外科系臨床医学 | |||||
出版者 | ||||||
出版者 | 弘前大学大学院医学研究科・弘前医学会 | |||||
資源タイプ | ||||||
値 | Article |