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Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System
http://hdl.handle.net/10129/3277
http://hdl.handle.net/10129/32778aa98133-17a0-4ebe-849f-165b6cc56c85
名前 / ファイル | ライセンス | アクション |
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HirosakiMedJ_61(1)_26.pdf (340.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-03-29 | |||||
タイトル | ||||||
タイトル | Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HER2/c-erbB-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | recombinant adenovirus vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cre/loxP system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cancer specifi c promoter | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 遺伝子/タンパク | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 組換えアデノウイルスベクター | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cre/loxP システム | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 癌特異的プロモーター | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nodagashira, Tatsuya
× Nodagashira, Tatsuya× Odagiri, Hiroki× Ikenaga, Shojiro-Kazunori× Maruyama, Masateru× Sato, Toshiyuki× Hakamada, Kenichi |
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著者(ヨミ) | ||||||
姓名 | ノダガシラ, タツヤ | |||||
著者(ヨミ) | ||||||
姓名 | オダギリ, ヒロキ | |||||
著者(ヨミ) | ||||||
姓名 | イケナガ, ショウジロウカズノリ | |||||
著者(ヨミ) | ||||||
姓名 | マルヤマ, マサテル | |||||
著者(ヨミ) | ||||||
姓名 | サトウ, トシユキ | |||||
著者(ヨミ) | ||||||
姓名 | ハカマダ, ケンイチ | |||||
著者別名 | ||||||
姓名 | 野田頭, 達也 | |||||
著者別名 | ||||||
姓名 | 小田桐, 弘毅 | |||||
著者別名 | ||||||
姓名 | 池永, 照史郎一期 | |||||
著者別名 | ||||||
姓名 | 丸山, 将輝 | |||||
著者別名 | ||||||
姓名 | 佐藤, 利行 | |||||
著者別名 | ||||||
姓名 | 袴田, 健一 | |||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
著者所属 | ||||||
Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tissue-specifi c promoter has been used for cancer-specifi c suicide gene therapy, but its transcriptional activity is relatively low. For more effi cient gene therapy of HER2-expressing tumor, a double adenovirus infection system was established, in which a ‘regulator’ vector carried Cre gene under the control of HER2 promoter and ‘target’ vectors carried target genes activated by Cre. We constructed a Cre recombinase expression vector, AxHER2Cre, for the ‘regulator’ vector. By the combination of this vector and AxCALNLZ, β-D-galactosidase was induced in 90% and 70% of MKN7 and MDA-MB-453, HER2‒overexpressing cell lines, but only about 20% and 10% of MKN28 and MCF7, low HER2-expressing cell lines. By the quantifi cation analysis, the β-galactosidase activities induced by this system were comparable to those by the combination of AxCANCre and AxCALNLZ. These results indicated that Cre/ loxP system under the regulation of HER2 promoter could induce effi cient gene expression, maintaining the HER2- expression specifi city. Breast cancer with HER2 overexpression is treated with trastuzumab. However, refractory or resitance of HER2 positive breast cancer against trastuzumab becomes a severe clinical problem, recently. This system seemed to be another therapeutic option. |
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引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 弘前医学. 61(1), 2010, p.26-34 | |||||
書誌情報 |
弘前医学 巻 61, 号 1, p. 26-34, 発行日 2010-03-25 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1721 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 491.6 | |||||
その他のタイトル | ||||||
その他のタイトル | Cre/loxP system を用いたHER2/neu 発現細胞に対する特異的かつ効率的遺伝子導入システムの開発 | |||||
タイトル(ヨミ) | ||||||
その他のタイトル | Cre/loxP system オ モチイタ HER2/neu ハツゲン サイボウ ニ タイスル トクイテキ カツ コウリツテキ イデンシ ドウニュウ システム ノ カイハツ | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 基礎医学 | |||||
出版者 | ||||||
出版者 | 弘前大学出版会 | |||||
資源タイプ | ||||||
値 | Article |