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Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1
http://hdl.handle.net/10129/5559
http://hdl.handle.net/10129/5559b20b1290-003b-4cbc-92de-445f88cf0f5d
名前 / ファイル | ライセンス | アクション |
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HirosakiMedJ_66(1)_22.pdf (2.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-05-25 | |||||
タイトル | ||||||
タイトル | Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Coronary spasm | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein kinase C | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phospholipase C-δ1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Calcium influx | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Calcium influx | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Acetylcholine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Murakami, Kazuo
× Murakami, Kazuo× Osanai, Tomohiro× Tanaka, Makoto× Kinjo, Takahiko× Tanno, Tomohiro× Narita, Ikuyo× Kawai, Misato× Tomita, Hirofumi× Okumura, Ken |
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著者所属 | ||||||
Department of Cardiology, Hirosaki University Graduate School of Medicine | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: We reported that enhanced phospholipase C (PLC)-δ1, which was detected in patients with coronary spasm, caused coronary spasm in mice. We investigated the role of protein kinase C( PKC) in acetylcholine (ACh)-induced Ca2+ influx under enhanced PLC-δ1 using human embryonic kidney( HEK)-293 cells. Methods and Results: Intracellular free Ca2+ concentration ([Ca2+]i) was measured by fura-2, and Ca2+ influx was evaluated by the increase in [Ca2+]i after addition of extracellular Ca2+. ACh-induced Ca2+ influx( nM) in HEK-293 cells was 21±2 in control HEK-293 cells, 52±6 in the cells with PLC-δ1 overexpression, and 75±9 in those with PLC-δ1 overexpression and PKC down-regulation (all p<0.05 among 3 groups). Ca2+ influx under treatment with nifedipine at 10-5M was 2.9±0.1 times higher in the cells with PLC-δ1 overexpression and 5.6±0.2 times higher in those with PLC-δ1 overexpression and PKC down-regulation compared with the control cells( all p<0.05 among 3 groups). Conclusions: ACh-induced Ca2+ influx was enhanced by PLC-δ1 overexpression, but was attenuated by PKC activation. PKC plays an important role in Ca2+ influx under enhanced PLC-δ1 in a negative feedback fashion. | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 弘前医学. 66, 2015, p.22-27 | |||||
書誌情報 |
弘前医学 巻 66, 号 1, p. 22-27, 発行日 2015-04-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0439-1721 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00211444 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 490 | |||||
NIIサブジェクト | ||||||
主題Scheme | Other | |||||
主題 | 医学 | |||||
出版者 | ||||||
出版者 | 弘前大学大学院医学研究科・弘前医学会 | |||||
資源タイプ | ||||||
値 | Article |