2026-03-12T02:04:41Z https://hirosaki.repo.nii.ac.jp/oai

oai:hirosaki.repo.nii.ac.jp:00003659 2023-05-15T10:26:25Z 557:1754615242943:561:644

Upregulation of Insulin-Like Growth Factor-1 Gene Expression in Cardiac Myocytes by Hypoxia : Mechanism for Its Cardioprotective Effect 低酸素による心筋細胞Insulin-like growth factor-1 遺伝子発現亢進の役割 : 心保護作用の機序について テイサンソ ニヨル シンキン サイボウ Insulin-like growth factor-1 イデンシ ハツゲン コウシン ノ ヤクワリ : シン ホゴ サヨウ ノ キジョ ニツイテ Kato, Chisato Osanai, Tomohiro Shibutani, Shuji Hanada, Kenji Okumura, Ken 491.3 内科系臨床医学 cardiac myocytes cardiac fibroblasts insulin-like growth factor-1 hypoxia application/pdf Insulin-like growth factor （IGF）-1 is known to exert benefi cial eff ects on the heart, but its source and function under hypoxia are unknown. We investigated the eff ect of hypoxia on IGF-1 expression and its role in the regeneration in the heart. Cardiac myocytes and fi broblasts obtained from neonate mice heart were cultured and exposed to hypoxia. mRNA of IGF-1, IGF-binding protein 3 （IGFBP3）, and vascular endothelial growth factor-A （VEGF-A） was measured by real-time PCR. In cardiac myocytes, IGF-1 mRNA was increased by 3.5±1.1 fold at 3 hours after hypoxia concomitantly with the increase in IGFBP3 and VEGF-A mRNA, and returned to the baseline at 24 hours. In contrast, IGF-1 mRNA in cardiac fi broblasts was unchanged by hypoxia, although VEGF-A mRNA was increased. To investigate the role of IGF-1 in the heart regeneration, we measured the gene expressions of stromal cell-derived factor-1（ SDF-1）, its receptor CXCR4, and matrix metalloproteinase（ MMP）-14 related to cell homing. In cardiac myocytes, SDF-1 and MMP-14 mRNA were increased at 3 hours after hypoxia and tended to be positively correlated with IGF-1 mRNA. These suggest that hypoxia increases IGF-1 expression in cardiac myocytes, and this endogenous IGF-1 may exert benefi cial eff ects on regeneration in the heart. 弘前医学. 60(1/2/3/4), 2009, p.36-44 弘前大学大学院医学研究科・弘前医学会 2009-03-31 eng journal article VoR http://hdl.handle.net/10129/1796 https://hirosaki.repo.nii.ac.jp/records/3659 AN00211444 0439-1721 弘前医学 60 1/2/3/4 36 44 https://hirosaki.repo.nii.ac.jp/record/3659/files/HirosakiMedJ_60_1-4_36.pdf application/pdf 340.6 kB 2018-03-13