2026-03-12T11:44:20Z https://hirosaki.repo.nii.ac.jp/oai

oai:hirosaki.repo.nii.ac.jp:00003610 2023-05-15T10:24:59Z 557:1754615242943:561:642

外因性プロテオグリカンが骨髄由来間葉系幹細胞の軟骨分化に与える影響 ガイインセイ プロテオグリカン ガ コツズイ ユライ カンヨウケイ カンサイボウ ノ ナンコツ ブンカ ニ アタエル エイキョウ Effect of Exogenous Proteoglycans on Chondrogenesis of Bone Marrow Mesenchymal Stem Cells Uesato, Ryoko Ishibashi, Yasuyuki Ohshika, Shusa Naraoka, Takuya Toh, Satoshi 494.7 外科系臨床医学 mesenchymal stem cells proteoglycan chondrogenesis 間葉系幹細胞 プロテオグリカン 軟骨分化 application/pdf Proteoglycans are one of the most important components of the extracellular matrix in the cartilage and the levels of proteoglycans. such as versican and aggrecan, increase during chondrogenesis. The purpose of this study was to investigate the effect of exogenous proteoglycans from salmon nasal cartilage on chondrogenesis of mesenchymal stem cells. Mesenchymal stem cells derived from bone marrow aspiration of rabbit femurs were induced to chondrogenic lineage using a pellet culture technique. Pellets were cultured in the medium with or without cell growth factors. with or without proteoglycans. or a combination of these agents. Pellets treated with cell growth factors became hypertrophic and showed lacuna formation. and synthesis of cartilage matrix was recognized histologically. The expression of type II collagen and aggrecan mRNA were decreased in pellets incubated with a combination of cell growth factors and proteoglycans, compared to those incubated with only cell growth factors. Exogenous proteoglycans may down-regulate the expression of cartilage-specific mRNA directly, or may interact with growth factors in the culture medium. As the increase of glycoprotein during chondrogenesis is important for determining the direction and degree of differentiation. exogenous proteoglycans may have a similar effect. 弘前医学. 59, 2008, p.98-103 departmental bulletin paper 弘前大学大学院医学研究科・弘前医学会 2008-03-31 VoR application/pdf 弘前医学 2/3/4 59 98 103 AN00211444 0439-1721 https://hirosaki.repo.nii.ac.jp/record/3610/files/AN00211444_59_98.pdf http://hdl.handle.net/10129/702 https://hirosaki.repo.nii.ac.jp/records/3610 eng