2026-03-12T11:18:35Z https://hirosaki.repo.nii.ac.jp/oai

oai:hirosaki.repo.nii.ac.jp:00003883 2023-05-15T09:58:52Z 557:1754615242943:561:659

The Cardiorespiratory Responses to Inhalation and Pentobarbital Anesthesia in the Mouse Chong, Han Ogata, Yoshiki Niwa, Hidetoshi Kushikata, Tetsuya Watanabe, Hiroyuki Imaizumi, Tadaatsu Hirota, Kazuyoshi Ono, Kyoichi Ohba, Takayoshi Murakami, Manabu 490 医学 Inhalation isoflurane anesthesia pentobarbital hemodynamic status blood gas Transgenic mice experiments have become increasingly popular to research human inherited disease. However, a number of Japanese researchers have difficulty with the selection of anesthesia, after the classification of ketamine, probably the most used anesthesia, as a narcotic drug in 2006. Therefore, we compared the effects of inhalation anesthesia （2% of isoflurane, sevoflurane and enflurane） and intraperitoneal pentobarbital anesthesia （50 mg/kg） on the electrocardiogram（ ECG） and blood oxygen saturation（ SPO2） of mice. With inhalation anesthesia, the heart rate（ HR） and SPO2 were within an acceptable range. In contrast, the HR significantly decreased after initiation of pentobarbital anesthesia, and gradually returned to a low rate. Importantly, pentobarbital anesthesia significantly lowered SPO2, and heart rate variability analysis showed unstable beat-to-beat intervals during pentobarbital anesthesia, suggesting that inhalation anesthesia is more suitable for evaluation of cardiorespiratory responses than pentobarbital anesthesia. During anesthesia, propranolol, a ????-adrenergic blocker, significantly decreased heart rate. Atropine, a parasympathetic blocker, also significantly increased heart rate. Our data suggest that inhalation 　anesthesia is suitable for cardiorespiratory analysis in mice. 弘前医学. 67, 2016, p.77-85 journal article 弘前大学大学院医学研究科・弘前医学会 2016-07-29 VoR application/pdf 弘前医学 1 67 77 85 AN00211444 0439-1721 https://hirosaki.repo.nii.ac.jp/record/3883/files/HirosakiMedJ_67(1)_77.pdf http://hdl.handle.net/10129/5923 https://hirosaki.repo.nii.ac.jp/records/3883 eng