{"created":"2024-04-29T06:13:54.836192+00:00","id":2000354,"links":{},"metadata":{"_buckets":{"deposit":"b41e7b1f-062f-47d5-9183-3d32d5fac6d8"},"_deposit":{"created_by":11,"id":"2000354","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000354"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:02000354","sets":["557:561:1713851803406"]},"author_link":[],"control_number":"2000354","item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"60","bibliographicPageStart":"52","bibliographicVolumeNumber":"74","bibliographic_titles":[{"bibliographic_title":"弘前医学","bibliographic_titleLang":"ja"},{"bibliographic_title":"Hirosaki Medical Journal","bibliographic_titleLang":"en"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Our previous study showed that chronic administration of diazepam （DZP） induces changes in gene expression in the hippocampus, cortex, and amygdala. Pathway analysis indicated that cell cycle was altered by chronic DZP treatment. These results raise the concern that chronic DZP administration provokes alterations in cell proliferation. The aim of this study is to elucidate the mechanism by which benzodiazepines（ BZDs） regulate cell cycle.\nCell growth curve analysis and MTT assays revealed that DZP treatment significantly reduced cell growth in the microglial cell line. Interestingly, cell growth was not affected by antagonizing the DZP target molecules, gammaaminobutyric acid （GABA） Type A receptor （GABAA-R）, and 18 kDa translocator protein （TSPO）. Similar to the results of DZP, other BZDs such as lorazepam （LZP） and midazolam （MDZ） showed a significant decrease in cell growth. In addition to the mouse microglial cell line results, mouse neuroblastoma cell line also showed an inhibition of cell growth. In contrast, only MDZ inhibited cell growth in human astrocytoma cell line, U373 cells. However, it is not clear whether these differences depend on species or cell types. Consequently, BZDs inhibit cell growth via GABAA-R- and TSPO-independent pathways.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科","subitem_publisher_language":"ja"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article","subitem_radio_language":"en"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2434-4656","subitem_source_identifier_type":"EISSN"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Neurophysiology, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shuji, Shimoyama","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tomonori, Furukawa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shinya, Ueno","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-04-29"}],"filename":"Hirosaki Med J 74-1-4_52.pdf","filesize":[{"value":"594 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hirosaki.repo.nii.ac.jp/record/2000354/files/Hirosaki Med J 74-1-4_52.pdf"},"version_id":"36e88fa8-4366-4a47-b687-337dc198d1d8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GABAA receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TSPO","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"benzodiazepine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell cycle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Benzodiazepine-induced cell growth inhibition via GABAA receptor- and TSPO-independent pathways","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Benzodiazepine-induced cell growth inhibition via GABAA receptor- and TSPO-independent pathways","subitem_title_language":"en"}]},"item_type_id":"3","owner":"11","path":["1713851803406"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-29"},"publish_date":"2024-04-29","publish_status":"0","recid":"2000354","relation_version_is_last":true,"title":["Benzodiazepine-induced cell growth inhibition via GABAA receptor- and TSPO-independent pathways"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-11-20T00:30:26.295314+00:00"}