{"created":"2025-01-09T06:48:39.135358+00:00","id":2000731,"links":{},"metadata":{"_buckets":{"deposit":"21e3f630-ee01-42e7-b2e6-108f04531372"},"_deposit":{"created_by":11,"id":"2000731","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000731"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:02000731","sets":["557:666:1733727873643"]},"author_link":[],"control_number":"2000731","item_1735019115310":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background\nAnalysis of CpG methylation is informative for cancer diagnosis. Previously, we developed a novel method to discriminate CpG methylation status in target DNA by blocking recombinase polymerase amplification (RPA), an isothermal DNA amplification technique, using methyl-CpG binding domain (MBD) protein 2 (MBD2). The method was named MBD protein interference-RPA (MBDi-RPA). In this study, MBDi-RPA was performed using methyl-CpG binding protein 2 (MeCP2), another MBD family protein, as the blocking agent.\nResults\nMBDi-RPA using MeCP2 detected low levels of CpG methylation, showing that it had higher sensitivity than MBDi-RPA using MBD2. We also developed real-time RPA, which enabled rapid analysis of DNA amplification without the need for laborious agarose gel electrophoresis and used\nit in combination with MBDi-RPA. We termed this method real-time MBDi-RPA. The method using MeCP2 could determine the abundance ratio of CpG-methylated target DNA simply and rapidly, although highly sensitive detection was challenging.\nSignificance and Novelty\nReal-time MBDi-RPA using MeCP2 could be potentially useful for estimating CpG methylation status in target DNA prior to more detailed analyses.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1735020952570":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.aca.2024.342486","subitem_relation_type_select":"DOI"}}]},"item_30001_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"embargoed access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_f1cf"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-05-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"342486","bibliographicVolumeNumber":"1302","bibliographic_titles":[{"bibliographic_title":"Analytica Chimica Acta","bibliographic_titleLang":"en"}]}]},"item_30001_creator2":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石戸谷, 美奈","creatorNameLang":"ja"}]}]},"item_30001_date_granted20":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-09-30"}]},"item_30001_degree_grantor21":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"弘前大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"11101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_30001_degree_name19":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"en"}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Author: Mina Ishidoya, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Citation: Analytica Chimica Acta, 1302:342486, 2024 May 8","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30001_dissertation_number18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2301号"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2025-01-10"}],"fileDate":[{"fileDateType":"Available","fileDateValue":"2025-05-08"}],"filename":"tdm_2301_ishidoya.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://hirosaki.repo.nii.ac.jp/record/2000731/files/tdm_2301_ishidoya.pdf"},"version_id":"9cbf3eb1-853d-4b2a-bea0-414e928d7246"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-09"}],"filename":"tdm_2301_ishidoya_a1.pdf","filesize":[{"value":"246 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"内容要旨","objectType":"abstract","url":"https://hirosaki.repo.nii.ac.jp/record/2000731/files/tdm_2301_ishidoya_a1.pdf"},"version_id":"2eb3b055-af1d-4eae-876c-90667ed1c6a3"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-09"}],"filename":"tdm_2301_ishidoya_a2.pdf","filesize":[{"value":"179 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"審査要旨","objectType":"abstract","url":"https://hirosaki.repo.nii.ac.jp/record/2000731/files/tdm_2301_ishidoya_a2.pdf"},"version_id":"8ce1a6c8-aee8-4385-a9ef-b1023f4626de"}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30001_publisher9":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V.","subitem_publisher_language":"en"}]},"item_30001_resource_type11":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_30001_rights5":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2024 Elsevier B.V. All rights reserved","subitem_rights_language":"en"}]},"item_30001_source_identifier16":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1873-4324","subitem_source_identifier_type":"EISSN"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CpG","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MBD","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MeCP2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RPA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MBDi-RPA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_title":"Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status","item_type_id":"40034","owner":"11","path":["1733727873643"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-01-09"},"publish_date":"2025-01-09","publish_status":"0","recid":"2000731","relation_version_is_last":true,"title":["Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-01-28T05:07:56.945003+00:00"}