{"created":"2025-01-10T04:08:17.345508+00:00","id":2000733,"links":{},"metadata":{"_buckets":{"deposit":"06d69663-34d2-4c90-a171-067703b3c66b"},"_deposit":{"created_by":11,"id":"2000733","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000733"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:02000733","sets":["557:666:1733727873643"]},"author_link":[],"control_number":"2000733","item_1735019115310":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocellular carcinoma (HCC) is the most predominant type of liver cancer and is frequently fatal. Alpha-fetoprotein, alpha-fetoprotein-L3, and protein induced by vitamin K absence or antagonist-II are used as biomarkers to diagnose HCC. However, these biomarkers are not highly specific, especially for early-stage HCC diagnosis; therefore, more specific biomarkers are needed. Recently, circular RNA (circRNA) biomarkers have been used to diagnose several intractable diseases. In this study, we sought to identify circRNA biomarkers for the specific diagnosis of HCC. To this end, we compared the expression levels of circRNAs in primary HCC and normal tissues using publicly available RNA-seq data. Our analysis  revealed that the expression levels of eight circRNAs were altered in primary HCC tissues compared with normal tissues. To confirm our findings, we examined the expression levels of selected circRNAs in HCC cell lines and normal hepatocytes. The expression level of hsa_circ_0001438, a circRNA that was downregulated in primary HCC, was lower in poorly and well-differentiated HCC cell lines than in normal hepatocytes. By contrast, the expression level of hsa_circ_0000417, which was increased in primary HCC, was strongly upregulated in a well-differentiated HCC cell line compared with normal hepatocytes. Thus, hsa_circ_0001438 and hsa_circ_0000417 might be potential biomarkers for the specific diagnosis of HCC. The experimental strategy described here, using publicly available RNA-seq data, is a useful and cost-effective method of identifying circRNA biomarkers.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1735020952570":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/iep.12457","subitem_relation_type_select":"DOI"}}]},"item_30001_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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祥子","creatorNameLang":"ja"}]}]},"item_30001_date_granted20":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-09-30"}]},"item_30001_degree_grantor21":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"弘前大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"11101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_30001_degree_name19":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Author: Sachiko Imanishi, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujii","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Citation: International Journal of Experimental Pathology 103(6):245-251, 2022 Dec","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_30001_dissertation_number18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2302号"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-08"}],"filename":"tdm_2505A_kuwamoto.pdf","filesize":[{"value":"776 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://hirosaki.repo.nii.ac.jp/record/2000733/files/tdm_2505A_kuwamoto.pdf"},"version_id":"a26b3462-820e-4c4b-8999-dd742cd05dd2"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-08"}],"filename":"tdm_2302_kuwamoto_a1.pdf","filesize":[{"value":"233 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"内容要旨","objectType":"abstract","url":"https://hirosaki.repo.nii.ac.jp/record/2000733/files/tdm_2302_kuwamoto_a1.pdf"},"version_id":"07838244-4955-4cb9-a82e-8562ae7fa205"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-08"}],"filename":"tdm_2302_kuwamoto_a2.pdf","filesize":[{"value":"172 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"審査要旨","objectType":"abstract","url":"https://hirosaki.repo.nii.ac.jp/record/2000733/files/tdm_2302_kuwamoto_a2.pdf"},"version_id":"e1f41759-43af-4e08-a59e-af3eac4272f2"}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30001_publisher9":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_30001_resource_type11":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_30001_rights5":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2022 Company of the International Journal of Experimental Pathology (CIJEP)","subitem_rights_language":"en"}]},"item_30001_source_identifier16":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1365-2613","subitem_source_identifier_type":"EISSN"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"circular RNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liver cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HCC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biomarker","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_title":"Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma","item_type_id":"40034","owner":"11","path":["1733727873643"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-01-10"},"publish_date":"2025-01-10","publish_status":"0","recid":"2000733","relation_version_is_last":true,"title":["Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-05-08T05:34:58.732188+00:00"}