{"created":"2025-03-25T08:42:59.693023+00:00","id":2000769,"links":{},"metadata":{"_buckets":{"deposit":"8c348e0b-f524-49f3-90bd-6ce02cd38ac9"},"_deposit":{"created_by":11,"id":"2000769","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000769"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:02000769","sets":["557:558:559"]},"author_link":[],"control_number":"2000769","item_1735019115310":{"attribute_name":"item_1735019115310","attribute_value_mlt":[{"subitem_description":"Glycosaminoglycan polysaccharides are components of animal extracellular matrices and regulate cell functions based on their various sulfation and epimerization pattern structures. The present study aimed to find glycosaminoglycan structures to promote neural differentiation. We investigated the effect of exogenous glycosaminoglycans with well-defined structures on the all-trans-retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells, which is an ideal model culture system for studying neural differentiation. We found that chondroitin sulfate E and heparin, but not any other glycosaminoglycans, upregulated the expressions of neural specific markers but not a grail specific marker. Chondroitin sulfate E was suggested to function during spheroid formation, however, equimolar concentration of its oligosaccharide did not show promotive effect on the neural differentiation. Another finding was that hyaluronan oligosaccharide mixture markedly downregulated the expressions of a myelin specific marker. These findings suggested that the specific sulfation pattern and/or chain length of exogenous added glycosaminoglycan is important to regulate neural differentiation and myelination.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1735020952570":{"attribute_name":"item_1735020952570","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2021.07.012","subitem_relation_type_select":"DOI"}}]},"item_30001_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_30001_bibliographic_information17":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-17","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"174","bibliographicPageStart":"169","bibliographicVolumeNumber":"570","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_30001_creator2":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ikuko, Kakizaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takayasu, Kobayashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shinri, Tamura","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hiroshi, Akagi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Keiichi, Takagaki","creatorNameLang":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30001_publisher9":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_30001_relation14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0006291X21010378?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_30001_resource_type11":{"attribute_name":"item_30001_resource_type11","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30001_rights5":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2021 Elsevier Inc. All rights reserved.","subitem_rights_language":"en"}]},"item_30001_source_identifier16":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"EISSN"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Neural differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycosaminoglycan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chondroitin sulfate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Heparin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hyaluronan","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of glycosaminoglycan structure on all-trans-retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_title":"Effect of glycosaminoglycan structure on all-trans-retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells","item_type_id":"40034","owner":"11","path":["559"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-03-25"},"publish_date":"2025-03-25","publish_status":"0","recid":"2000769","relation_version_is_last":true,"title":["Effect of glycosaminoglycan structure on all-trans-retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-03-27T00:59:33.013183+00:00"}