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Role of CD34 in calcification of human aortic valve interstitial cells from patients with aortic valve stenosis
http://hdl.handle.net/10129/0002000926
http://hdl.handle.net/10129/000200092643796960-b54b-4c4a-a111-6e14fd8e3e7a
| 名前 / ファイル | ライセンス | アクション |
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本文 (2.7 MB)
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内容要旨 (227 KB)
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審査要旨 (117 KB)
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| アイテムタイプ | リポジトリ登録用アイテムタイプ（シンプル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-06-13 | |||||||
| タイトル | ||||||||
| タイトル | Role of CD34 in calcification of human aortic valve interstitial cells from patients with aortic valve stenosis | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | ja | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Calcified aortic valve stenosis | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Aortic valve interstitial cells | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | CD34Tenascin X | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Calcification | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
門, 士虎
× 門, 士虎
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Various osteogenic factors are involved in ectopic human aortic valve calcification; however, the key cell species involved in calcification remains unclear. In a previous study, we reported that mesenchymal stem (CD73, 90, 105) and endothelial (VEGFR2) cell markers are positive in almost all human aortic valve interstitial cells (HAVICs) obtained from a patient with calcified aortic valve stenosis (CAVS). Further, CD34-negative HAVICs are highly sensitive to calcification stimulations. Here, we aimed to pathophysiologically clarify the role of CD34 in HAVICs obtained from individual patients with severe CAVS. A DNA microarray between CD34-positive and CD34-negative HAVICs, separated by fluorescence-activated cell sorting, indicated that tenascin X (TNX) mRNA expression significantly decreased in CD34-negative cells. Furthermore, the inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-1β significantly downregulated CD34 expression in HAVICs. TGF-β, a key cytokine of endothelial-mesenchymal transition, did not affect HAVIC calcification. CD34 overexpression strongly inhibited TNF-α- and IL-1β-induced calcification and maintained TNX mRNA expression. These results suggest one possibility that CD34 is an inhibitory regulator of valve calcification. Furthermore, TNF-α- and IL-1β-induced CD34 downregulation in HAVICs contributes to HAVIC calcification by downregulating TNX protein expression. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Author(s)：Shihu Men , Zaiqiang Yu , Xu Liu , Kazuyuki Daitoku , Mayuki Tachizaki , Shogo Kawaguchi , Tadaatsu Imaizumi , Masahito Minakawa , Kazuhiko Seya 掲載誌：Journal of Pharmacological Sciences（156(3), 196-207）[日本薬理学会] 出版社：ELSEVIER © 2024 . This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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| 言語 | en | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 1347-8613 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1016/j.jphs.2024.09.002 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | NA | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 言語 | ja | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関名 | 弘前大学 | |||||||
| 言語 | ja | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2025-03-24 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第2310号 | |||||||
