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Expression of ISG60 is induced by TLR3 signaling in BEAS-2B bronchial epithelial cells: Possible involvement in CXCL10 expression
http://hdl.handle.net/10129/0002000931
http://hdl.handle.net/10129/00020009316d970956-3331-4fb3-8a86-78e85f3fb906
| 名前 / ファイル | ライセンス | アクション |
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本文 (3.3 MB)
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内容要旨 (186 KB)
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審査要旨 (257 KB)
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| アイテムタイプ | リポジトリ登録用アイテムタイプ（シンプル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-06-16 | |||||||
| タイトル | ||||||||
| タイトル | Expression of ISG60 is induced by TLR3 signaling in BEAS-2B bronchial epithelial cells: Possible involvement in CXCL10 expression | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | bronchial epithelial cells | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | CXCL10 | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | IFN-β | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | ISG60 | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | TLR3 | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
田中, 佑典
× 田中, 佑典
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Viral infections in the respiratory tract are common, and, in recent years, severe acute respiratory syndrome coronavirus 2 outbreaks have highlighted the effect of viral infections on antiviral innate immune and inflammatory reactions. Specific treatments for numerous viral respiratory infections have not yet been established and they are mainly treated symptomatically. Therefore, understanding the details of the innate immune system underlying the airway epithelium is crucial for the development of new therapies. The present study aimed to investigate the function and expression of interferon (IFN)-stimulated gene (ISG)60 in non-cancerous bronchial epithelial BEAS-2B cells exposed to a Toll-like receptor 3 agonist. BEAS-2B cells were treated with a synthetic TLR3 ligand, polyinosinic-polycytidylic acid (poly IC). The mRNA and protein expression levels of ISG60 were analyzed using reverse transcription-quantitative PCR and western blotting, respectively. The levels of C-X-C motif chemokine ligand 10 (CXCL10) were examined using an enzyme-linked immunosorbent assay, and the effects of knockdown of IFN-β, ISG60 and ISG56 were examined using specific small interfering RNAs. Notably, ISG60 expression was increased in proportion to poly IC concentration, and recombinant human IFN-β also induced ISG60 expression. By contrast, knockdown of IFN-β and ISG56 decreased ISG60 expression, and ISG60 knockdown reduced CXCL10 and ISG56 expression. These findings suggested that ISG60 is partly implicated in CXCL10 expression and that ISG60 may serve a role in the innate immune response of bronchial epithelial cells. The present study highlights ISG60 as a potential target for new therapeutic strategies against viral infections in the airway. | |||||||
| 言語 | en | |||||||
| 書誌情報 |
en : MOLECULAR MEDICINE REPORTS 巻 30, 号 3 |
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| ISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 1791-3004 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.3892/mmr.2024.13276 | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 出版者 | ||||||||
| 出版者 | Spandidos Publications | |||||||
| 言語 | en | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 言語 | ja | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関名 | 弘前大学 | |||||||
| 言語 | en | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2025-03-24 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第2315号 | |||||||
