| アイテムタイプ |
リポジトリ登録用アイテムタイプ（シンプル）(1) |
| 公開日 |
2025-10-15 |
| タイトル |
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|
タイトル |
Rapid method for plasmid DNA recombination (Murakami-system) |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pcr |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Plasmid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dna |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Competent cell |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
Murakami, M Agnieszka
Yonekura, Manabu
Nagatomo, Katsuhiro
Niwa, Yasutaka
Itagaki, Shirou
Murakami, Manabu
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged. In the present study, we introduced rapid method for plasmid DNA recombination, which we named “Murakami-system”, to complete the experiments in under 33 h. For this purpose, we selected the following: PCR amplification with 25 cycles and E. coli strain with rapid growth (incubation time of 6–8 h). In addition, we selected rapid plasmid DNA purification (mini-prep; ∼10 min) and rapid restriction enzyme incubation (20 min). This recombination system enabled rapid plasmid DNA recombination within 24–33 h, which could be useful in various fields. We also established a 1-day method for competent cell preparation. Our rapid recombination system allowed several sessions of plasmid DNA recombination to be performed every week, which improves the functional analysis of various genes.
•“Rapid method for plasmid DNA recombination (Murakami-system).
•E. coli strain with rapid growth (incubation time of 6–8 h).
•Combination of rapid protocols (PCR, electrophoresis, DNA purification, ligation, and mini-prep) enabled plasmid DNA recombination within 24–33 h. |
|
言語 |
en |
| 書誌情報 |
en : MethodsX
巻 10,
p. 102167,
発行日 2023
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2215-0161 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.mex.2023.102167 |
| 権利情報 |
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|
権利情報 |
© 2023 The Authors. Published by Elsevier B.V. This article is published under a Creative Commons CC-BY license, permitting unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. No permission is required to reuse this article. |
|
言語 |
en |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1-s2.0-S221501612300167X-main.pdf (706 KB)
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