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Isogenic Transplantation of Hybrid Artificial Pleural Tissue Consisting of Rat Cells and Polyglycolic Acid Nanofiber Sheet Induces Restoration of Mesothelial Defects in Rat Model
http://hdl.handle.net/10129/0002001875
http://hdl.handle.net/10129/0002001875d23fc6a2-ba6e-45cb-84e3-eb00dee666f2
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審査要旨 (134 KB)
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| アイテムタイプ | リポジトリ登録用アイテムタイプ（シンプル）(1) | |||||||||
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| 公開日 | 2025-11-26 | |||||||||
| タイトル | ||||||||||
| タイトル | Isogenic Transplantation of Hybrid Artificial Pleural Tissue Consisting of Rat Cells and Polyglycolic Acid Nanofiber Sheet Induces Restoration of Mesothelial Defects in Rat Model | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | ラット細胞とポリグリコール酸ナノファイバーシートで構成されたハイブリット人工胸膜の中皮欠損モデルへの同系移植 | |||||||||
| 言語 | ja | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | artificial pleural tissue | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | cell-accumulation method | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | mesothelial cells | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | polyglycolic acid nanofiber sheet | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | tissue engineering | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 著者 |
谷, 建吾
× 谷, 建吾
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background Impairment of the visceral pleura following thoracic surgery often leads to air leaks and intrathoracic adhesions. For preventing such complications, mesothelial cell proliferation at the pleural defects can be effective. To develop new materials for pleural defects restoration, we constructed a hybrid artificial pleural tissue (H-APLT) combining polyglycolic acid (PGA) nanofiber sheets with a three-dimensional culture of mesothelial cells and fibroblasts and evaluated its therapeutic efficacy in a rat pleural defect model. Methods After rat lungs were harvested, pleural mesothelial cells and lung fibroblasts were cultured separately. To construct H-APLT, the cells were then coated with multiple layers of fibronectin and gelatin, followed by a single layer of mesothelial cells on top of multiple layers of fibroblasts accumulated onto a collagen-coated PGA nanofiber sheet. Left lateral thoracotomy was performed, and H-APLTs were transplanted into a rat model with pleural defects (N = 8). After 2–12 weeks of transplantation, lung resection and histological analyses were performed. Results H-APLTs exhibited a pleural structure with a highly integrated mesothelial layer in vitro. After transplantation, all eight rats survived until sacrifice. At 12 weeks post-transplantation, the mesothelial layer on the lung surface was observed to be without defects with no intrathoracic adhesions detected. Conclusion Successful isogenic engraftment of H-APLTs was achieved in a rat model of pleural defects. The combination of accumulated fibroblasts and collagen-coated PGA nanofiber sheets contributed to the maintenance of the mesothelial layer's structure and function, potentially preventing air leaks and intrathoracic adhesions. |
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| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Author(s): Tani, Kengo ; Kimura, Daisuke ; Asano, Yoshiya ; Song, Cheng-Yang ; Shimoda, Hiroshi ; Minakawa, Masahito | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
en : Artificial Organs 巻 49, 号 5, p. 778-789, 発行日 2025-05 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 1525-1594 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1111/aor.14947 | |||||||||
| 権利情報 | ||||||||||
| 権利情報 | © 2025 The Author(s). Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | |||||||||
| 言語 | en | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 | ||||||||||
| 出版者 | Wiley | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士（医学） | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関名 | 弘前大学 | |||||||||
| 言語 | ja | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2025-09-30 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第2343号 | |||||||||
