| アイテムタイプ |
リポジトリ登録用アイテムタイプ（シンプル）(1) |
| 公開日 |
2025-12-02 |
| タイトル |
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タイトル |
Impact of intermittent high-dose radon exposures on lung epithelial cells: proteomic analysis and biomarker identification |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Subsomwong Phawinee
Kranrod Chutima
Sakai Yuna
Asano Krisana
Nakane Akio
Tokonami Shinji
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Lung cancer is the most prevalent cancer worldwide, and radon exposure is ranked as the second risk factor after cigarette smoking. It has been reported that radon induces deoxyribonucleic acid damage and oxidative stress in cells. However, the protein profile and potential biomarkers for early detection of radon-induced lung cancer remain unknown. In this study, we aimed to investigate the effects of intermittent high-dose radon exposure on lung epithelial cells, analyze protein profiles and identify potential biomarkers for diagnosis of radon-related lung cancer. Human lung epithelial cells (A549) were exposed to radon (1000 Bq/m3) for 30 min daily for 7 days. Cell viability was measured using the WST-1 assay, and liquid chromatography–mass spectrometry proteomic analysis was performed. Differentially expressed proteins and gene ontology (GO) enrichment were analyzed. Our findings showed that intermittent high-radon exposure reduced A549 cell viability over time. Proteomic analysis identified proteins associated with stressed-induced apoptosis, mitochondrial adaptation, nuclear integrity and lysosomal degradation. These proteins are related to catabolism, stress response, gene expression and metabolic processes in the biological process of GO analysis. We highlighted specific proteins, including AKR1B1, CDK2, DAPK1, PRDX1 and ALHD2 with potential as biomarkers for radon-related lung cancer. In summary, intermittent high-dose radon exposure affects cellular adaptions of lung epithelial cells including stress-induced apoptosis, mitochondrial dysfunctions and immune regulation. The identified proteins may serve as diagnostic biomarkers or therapeutic targets for radon-related lung cancer. |
|
言語 |
en |
| 書誌情報 |
en : Journal of Radiation Research
巻 66,
号 2,
p. 107-114,
発行日 2025
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| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/jrr/rraf010 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0449-3060 |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1349-9157 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/jrr/rraf010 |
| 権利情報 |
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権利情報 |
© The Author(s) 2025. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. |
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言語 |
en |
| 権利情報 |
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権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
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出版者 |
Oxford University Press |
|
言語 |
en |
rraf010.pdf (926 KB)
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