@article{oai:hirosaki.repo.nii.ac.jp:00003548,
 author = {Mariya, Yasushi and Streffer, Christian and Abe, Yoshinao},
 issue = {4},
 journal = {弘前医学},
 month = {Mar},
 note = {To investigate the applicability of the cytokinesis-block micronucleus（MN）assay to assess combined effects of irradiation and cisplatin, the quantitative relationship between the induced MN frequency and clonogenic survival by colony forming assay was estimated using the human squamous cell carcinoma cell line PECA 4197. First, the MN frequencies induced by cisplatin treatment alone, at various concentrations, were examined and compared with the corresponding clonogenic survival. Secondly, cells were irradiated with and without cisplatin pretreatment and compared. Treatment with cisplatin alone exhibited a dose-dependent increase of induced MN frequency, at concentrations of 0-1.0μg/ml and a treatment time of 180min, with a significant relation to dose-dependently decreased clonogenic survival. Irradiation after cisplatin pretreatment (0.25μg/ml, 180min) resulted in a higher MN frequency than either irradiation or cisplatin alone and also than simple addition of the single effects after 2 and 4Gy X-rays, suggesting a chemoradiosensitization. There was a significant correlation of the induced MN frequency with clonogenic survival for the combined effects of irradiation and cisplatin as well as for cisplatin or irradiation treatment alone. These results suggest that the induced MN frequency is a reliable quantitative measure to assess cell damage due to single or combined treatment with irradiation and cisplatin, Providing encouragement for clinical use of the cytokinesis-block MN assay as a predictive tool to optimize and individualize multidisciplinary tumor therapy., 弘前医学. 50(4), 1999, p.215-222},
 pages = {215--222},
 title = {Utility of the Cytokinesis－block Micronucleus Assay to Assess Combined Effects of Irradiation and Cisplatin},
 volume = {50},
 year = {1999}
}