{"created":"2023-05-15T09:02:41.001935+00:00","id":3548,"links":{},"metadata":{"_buckets":{"deposit":"c563b70c-f5c5-466d-897a-cd761701c5c1"},"_deposit":{"created_by":3,"id":"3548","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3548"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003548","sets":["557:561:626"]},"author_link":["10544","10780","10805","10789","10562","10771","10553"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"放射線およびシスプラチン併用効果の評価における細胞分裂阻害徽小核形成試験の有用性"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ホウシャセン オヨビ シスプラチン ヘイヨウ コウカ ノ ヒョウカ ニ オケル サイボウ ブンレツ ソガイ ビショウカク ケイセイ シケン ノ ユウヨウセイ"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"222","bibliographicPageStart":"215","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the applicability of the cytokinesis-block micronucleus（MN）assay to assess combined effects of irradiation and cisplatin, the quantitative relationship between the induced MN frequency and clonogenic survival by colony forming assay was estimated using the human squamous cell carcinoma cell line PECA 4197. First, the MN frequencies induced by cisplatin treatment alone, at various concentrations, were examined and compared with the corresponding clonogenic survival. Secondly, cells were irradiated with and without cisplatin pretreatment and compared. Treatment with cisplatin alone exhibited a dose-dependent increase of induced MN frequency, at concentrations of 0-1.0μg/ml and a treatment time of 180min, with a significant relation to dose-dependently decreased clonogenic survival. Irradiation after cisplatin pretreatment (0.25μg/ml, 180min) resulted in a higher MN frequency than either irradiation or cisplatin alone and also than simple addition of the single effects after 2 and 4Gy X-rays, suggesting a chemoradiosensitization. There was a significant correlation of the induced MN frequency with clonogenic survival for the combined effects of irradiation and cisplatin as well as for cisplatin or irradiation treatment alone. These results suggest that the induced MN frequency is a reliable quantitative measure to assess cell damage due to single or combined treatment with irradiation and cisplatin, Providing encouragement for clinical use of the cytokinesis-block MN assay as a predictive tool to optimize and individualize multidisciplinary tumor therapy.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 50(4), 1999, p.215-222","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"10553","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マリヤ, ヤスシ"}]},{"nameIdentifiers":[{"nameIdentifier":"10789","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アベ, ヨシナオ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"10544","nameIdentifierScheme":"WEKO"}],"names":[{"name":"真里谷, 靖"}]},{"nameIdentifiers":[{"nameIdentifier":"10771","nameIdentifierScheme":"WEKO"}],"names":[{"name":"阿部, 由直"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学医学部・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"494.54","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"外科系臨床医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Institut fur Medizinische Strahlenbiologie，Universitats－klinikum Essen"},{"subitem_text_value":"Department of Radiology，Hirosaki University, Schoolof Medicine"},{"subitem_text_value":"ドイツ・エッセン大学医学放射線生物学研究所"},{"subitem_text_value":"弘前大学医学部放射線医学講座"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mariya, Yasushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Streffer, Christian"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Yoshinao"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_50_215.pdf","filesize":[{"value":"765.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_50_215.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3548/files/HirosakiMedJ_50_215.pdf"},"version_id":"a5eeeafc-9269-4d29-884e-b1feaa5f2fc5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cisplatin","subitem_subject_scheme":"Other"},{"subitem_subject":"irradiation","subitem_subject_scheme":"Other"},{"subitem_subject":"chemoradiosensitization","subitem_subject_scheme":"Other"},{"subitem_subject":"micronucleus assay","subitem_subject_scheme":"Other"},{"subitem_subject":"clonogenic survival","subitem_subject_scheme":"Other"},{"subitem_subject":"シスプラチン","subitem_subject_scheme":"Other"},{"subitem_subject":"放射線照射","subitem_subject_scheme":"Other"},{"subitem_subject":"化学放射線増感","subitem_subject_scheme":"Other"},{"subitem_subject":"微小核形成試験","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞生存率","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Utility of the Cytokinesis－block Micronucleus Assay to Assess Combined Effects of Irradiation and Cisplatin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Utility of the Cytokinesis－block Micronucleus Assay to Assess Combined Effects of Irradiation and Cisplatin"}]},"item_type_id":"3","owner":"3","path":["626"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-18"},"publish_date":"2013-10-18","publish_status":"0","recid":"3548","relation_version_is_last":true,"title":["Utility of the Cytokinesis－block Micronucleus Assay to Assess Combined Effects of Irradiation and Cisplatin"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:03:00.137370+00:00"}