{"created":"2023-05-15T09:02:43.212129+00:00","id":3586,"links":{},"metadata":{"_buckets":{"deposit":"1ee1c37d-2c56-4378-95e8-db5d433774ed"},"_deposit":{"created_by":3,"id":"3586","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3586"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003586","sets":["557:561:638"]},"author_link":["10950","11304","10960","11307","11302","11300","10956","11260","11250","11255","10951","11309","11312","10946","10955"],"item_5_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"低用量末梢血幹細胞と脾細胞混合移入による非致死的照射マウスへのキメリズムの導入"}]},"item_5_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"テイヨウリョウ マッショウ ケッセン サイボウ ト ヒサイボウ コンゴウ イニュウ ニヨル ヒチシテキ ショウシャ マウス エノ キメリズム ノ ドウニュウ"}]},"item_5_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"26","bibliographicPageStart":"17","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_5_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: We previously demonstrated that a high level of chimerism was induced by low dose of bone marrow cells (BMCs) combined with splenocytes (SPLCs), while the same dose of BMCs alone was insufficient for tolerance induction. In the present study, we investigated whether stable mixed chimerism and donor specific tolerance could be established in sublethally irradiated mice following intravenous injection of various dosages of fully allogeneic peripheral blood stem cells (PBSCs). In addition, validity of combination of donor splenocytes in the peripheral blood stem cell transplantation (PBSCT) as well as in bone marrow transplantation for tolerance induction were examined. Methods: Sublethally irradiated C57BL/6 recipient mice were injected 10×10^6 PBSCs mixed with various numbers of granulocyte-colony-stimulating factor (G-CSF)-mobilized SPLCs harvested from BALB/c donor mice. Three months later, the degree of chimerism in peripheral blood lymphocytes and in SPLCs was examined by FACS analysis. Mixed lymphocyte responses (MLR) and skin grafting were performed to confirm donor-specific tolerance. Results: Recipients receiving 10×10^6 PBSCs mixed with 30×10^6 G-CSF-mobilized SPLCs showed a higher level of chimerism. High percentage chimeric mice all showed donor specific tolerance in vitro, MLR and in vivo, and skin grafting. Conclusions: PBSCT mixed with SPLCs was proven to be useful for induction of donor-specific tolerance. Admixture of SPLCs reduces the amount of PBSCs needed for induction of tolerance.","subitem_description_type":"Abstract"}]},"item_5_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 57(1), 2005, p.17-26","subitem_description_type":"Other"}]},"item_5_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11309","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハシモト, ナオキ"}]},{"nameIdentifiers":[{"nameIdentifier":"11255","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ナルミ, シュンジ"}]},{"nameIdentifiers":[{"nameIdentifier":"10951","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イタバシ, ユキヒロ"}]},{"nameIdentifiers":[{"nameIdentifier":"11312","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハカマダ, ケンイチ"}]},{"nameIdentifiers":[{"nameIdentifier":"10955","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ササキ, ムツオ"}]}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11304","nameIdentifierScheme":"WEKO"}],"names":[{"name":"橋本, 直樹"}]},{"nameIdentifiers":[{"nameIdentifier":"11250","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鳴海, 俊治"}]},{"nameIdentifiers":[{"nameIdentifier":"10946","nameIdentifierScheme":"WEKO"}],"names":[{"name":"板橋, 幸弘"}]},{"nameIdentifiers":[{"nameIdentifier":"11307","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"257","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/257_ja.html"}],"names":[{"name":"袴田, 健一"}]},{"nameIdentifiers":[{"nameIdentifier":"10950","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐々木, 睦男"}]}]},"item_5_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学医学部・弘前医学会"}]},"item_5_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_5_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"494.28","subitem_subject_scheme":"NDC"}]},"item_5_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"外科系臨床医学","subitem_subject_scheme":"Other"}]},"item_5_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Second Department of Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Second Department of Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Second Department of Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Second Department of Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Second Department of Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"弘前大学医学部第二外科"},{"subitem_text_value":"弘前大学医学部第二外科"},{"subitem_text_value":"弘前大学医学部第二外科"},{"subitem_text_value":"弘前大学医学部第二外科"},{"subitem_text_value":"弘前大学医学部第二外科"}]},"item_5_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hashimoto, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narumi, Shunji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Itabashi, Yukihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hakamada, Kenichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sasaki, Mutsuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"KJ00004529548.pdf","filesize":[{"value":"641.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004529548.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3586/files/KJ00004529548.pdf"},"version_id":"9b5633f3-6762-47b9-8d95-fd539ca2f986"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"末梢血幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"キメリズム","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_scheme":"Other"},{"subitem_subject":"脾細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫寛容","subitem_subject_scheme":"Other"},{"subitem_subject":"peripheral blood stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"chimerism","subitem_subject_scheme":"Other"},{"subitem_subject":"mice","subitem_subject_scheme":"Other"},{"subitem_subject":"splenocytes","subitem_subject_scheme":"Other"},{"subitem_subject":"tolerance","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Allogeneic Chimerism Induced by Low Dose of Peripheral Blood Stem Cells Combined with Splenocytes in Sublethally Irradiated Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Allogeneic Chimerism Induced by Low Dose of Peripheral Blood Stem Cells Combined with Splenocytes in Sublethally Irradiated Mice"}]},"item_type_id":"5","owner":"3","path":["638"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-05-01"},"publish_date":"2007-05-01","publish_status":"0","recid":"3586","relation_version_is_last":true,"title":["Allogeneic Chimerism Induced by Low Dose of Peripheral Blood Stem Cells Combined with Splenocytes in Sublethally Irradiated Mice"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:25:40.592535+00:00"}