{"created":"2023-05-15T09:02:43.414337+00:00","id":3589,"links":{},"metadata":{"_buckets":{"deposit":"79247b31-d96f-41e1-a2bb-a07e2e2514ba"},"_deposit":{"created_by":3,"id":"3589","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3589"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003589","sets":["557:561:639"]},"author_link":["11336","11237","11244","11245","11341","11331","11238","11335","11332","11240","11246","11236","11340","11241","11242"],"item_5_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト黄色靭帯の細胞外マトリックスを構成するプロテオグリカン"}]},"item_5_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ヒト オウショク ジンタイ ノ サイボウガイ マトリックス オ コウセイスル プロテオグリカン"}]},"item_5_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2/3/4","bibliographicPageEnd":"58","bibliographicPageStart":"49","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_5_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Three types of proteoglycans (PG-I, II and III) present in the human yellow ligament extracellular matrix were separated using a combination of CsCl isopycnic density gradient centrifugation, DEAE-Sephacel ion-exchange chromatography, gel-filtration on Sepharose CL-4B and CL-2B, and Octyl-Sepharose CL-4B chromatography. Proteoglycan-I was a high-molecular-weight proteoglycan, which was excluded by Sepharose CL-4B, and its molecular weight, estimated using sodium dedecyl sulfate-polyacrylamide gel electrophoresis, was over 350,000. The glycosaminoglycan chain of PG-I was composed of chondroitin 6-sulfate, and PG-I appeared to be an aggrecan-type proteoglycan. Proteoglycan-II and III were low-molecular-weight proteoglycans with molecular weights of 190,000 and 105,000, respectively. The core protein sizes of PG-II and III were similar, 44,000, but anti-human decorin antibody reacted with PG-III, not with PG-II. The glycosaminoglycan chain of PG-II contained dermatan sulfate and chondroitin 6-sulfate, whereas that of PG-III contained the former only. In the light of these results, PG-II and III were suggested to be biglycan- and decorin-type proteoglycans, respectively. The major proteoglycan in the human yellow ligament extracellular matrix was PG-III.","subitem_description_type":"Abstract"}]},"item_5_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 57(2/3/4), 2006, p.49-58","subitem_description_type":"Other"}]},"item_5_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11335","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ナリタ, ホズミ"}]},{"nameIdentifiers":[{"nameIdentifier":"11336","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タケダ, ユウスケ"}]},{"nameIdentifiers":[{"nameIdentifier":"11244","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハラタ, セイコウ"}]},{"nameIdentifiers":[{"nameIdentifier":"11245","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タカガキ, ケイイチ"}]},{"nameIdentifiers":[{"nameIdentifier":"11246","nameIdentifierScheme":"WEKO"}],"names":[{"name":"エンドウ, マサヒコ"}]}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11340","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Hozumi"}]},{"nameIdentifiers":[{"nameIdentifier":"11341","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takeda, Yusuke"}]},{"nameIdentifiers":[{"nameIdentifier":"11236","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Harata, Seiko"}]},{"nameIdentifiers":[{"nameIdentifier":"11237","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takagaki, Keiichi"}]},{"nameIdentifiers":[{"nameIdentifier":"11238","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Endo, Masahiko"}]}]},"item_5_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学医学部・弘前医学会"}]},"item_5_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_5_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.1","subitem_subject_scheme":"NDC"}]},"item_5_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"基礎医学","subitem_subject_scheme":"Other"}]},"item_5_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biochemistry, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Biochemistry, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Biochemistry, Hirosaki University School of Medicine"},{"subitem_text_value":"弘前大学医学部第一生化学教室"},{"subitem_text_value":"弘前大学医学部整形外科学教室"},{"subitem_text_value":"弘前大学医学部整形外科学教室"},{"subitem_text_value":"弘前大学医学部第一生化学教室"},{"subitem_text_value":"弘前大学医学部第一生化学教室"}]},"item_5_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"成田, 穂積"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"武田, 裕介"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"原田, 征行"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高垣, 啓一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"遠藤, 正彦"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"KJ00004529552.pdf","filesize":[{"value":"847.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004529552.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3589/files/KJ00004529552.pdf"},"version_id":"4c960650-05d8-4af8-ba83-a2aa277a029b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ヒト黄色靭帯","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞外マトリックス","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオグリカン","subitem_subject_scheme":"Other"},{"subitem_subject":"デコリン","subitem_subject_scheme":"Other"},{"subitem_subject":"バイグリカン","subitem_subject_scheme":"Other"},{"subitem_subject":"human yellow ligament","subitem_subject_scheme":"Other"},{"subitem_subject":"proteoglycan","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular matrix","subitem_subject_scheme":"Other"},{"subitem_subject":"decorin","subitem_subject_scheme":"Other"},{"subitem_subject":"biglycan","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Constituent Proteoglycans of the Human Yellow Ligament Extracellular Matrix","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Constituent Proteoglycans of the Human Yellow Ligament Extracellular Matrix"}]},"item_type_id":"5","owner":"3","path":["639"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-05-02"},"publish_date":"2007-05-02","publish_status":"0","recid":"3589","relation_version_is_last":true,"title":["Constituent Proteoglycans of the Human Yellow Ligament Extracellular Matrix"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T09:47:32.223272+00:00"}