{"created":"2023-05-15T09:02:44.602662+00:00","id":3610,"links":{},"metadata":{"_buckets":{"deposit":"613fa2c5-4fe6-47ca-8a3a-3401eef8e79b"},"_deposit":{"created_by":3,"id":"3610","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3610"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003610","sets":["557:561:642"]},"author_link":["10706","11711","10707","11713","11714","11717","11707","11712","11705","10708","11718","11706","11716","11709","11708"],"item_5_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"外因性プロテオグリカンが骨髄由来間葉系幹細胞の軟骨分化に与える影響"}]},"item_5_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ガイインセイ プロテオグリカン ガ コツズイ ユライ カンヨウケイ カンサイボウ ノ ナンコツ ブンカ ニ アタエル エイキョウ"}]},"item_5_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2/3/4","bibliographicPageEnd":"103","bibliographicPageStart":"98","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_5_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Proteoglycans are one of the most important components of the extracellular matrix in the cartilage and the levels of proteoglycans. such as versican and aggrecan, increase during chondrogenesis. The purpose of this study was to investigate the effect of exogenous proteoglycans from salmon nasal cartilage on chondrogenesis of mesenchymal stem cells. Mesenchymal stem cells derived from bone marrow aspiration of rabbit femurs were induced to chondrogenic lineage using a pellet culture technique. Pellets were cultured in the medium with or without cell growth factors. with or without proteoglycans. or a combination of these agents. Pellets treated with cell growth factors became hypertrophic and showed lacuna formation. and synthesis of cartilage matrix was recognized histologically. The expression of type II collagen and aggrecan mRNA were decreased in pellets incubated with a combination of cell growth factors and proteoglycans, compared to those incubated with only cell growth factors. Exogenous proteoglycans may down-regulate the expression of cartilage-specific mRNA directly, or may interact with growth factors in the culture medium. As the increase of glycoprotein during chondrogenesis is important for determining the direction and degree of differentiation. exogenous proteoglycans may have a similar effect.","subitem_description_type":"Abstract"}]},"item_5_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 59, 2008, p.98-103","subitem_description_type":"Other"}]},"item_5_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11709","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ウエサト, リョウコ"}]},{"nameIdentifiers":[{"nameIdentifier":"10707","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イシバシ, ヤスユキ"}]},{"nameIdentifiers":[{"nameIdentifier":"11711","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オオシカ, シュウサ"}]},{"nameIdentifiers":[{"nameIdentifier":"11712","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ナラオカ, タクヤ"}]},{"nameIdentifiers":[{"nameIdentifier":"11713","nameIdentifierScheme":"WEKO"}],"names":[{"name":"トウ, サトシ"}]}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11714","nameIdentifierScheme":"WEKO"}],"names":[{"name":"上里, 涼子"}]},{"nameIdentifiers":[{"nameIdentifier":"10706","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"263","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/263_ja.html"}],"names":[{"name":"石橋, 恭之"}]},{"nameIdentifiers":[{"nameIdentifier":"11716","nameIdentifierScheme":"WEKO"}],"names":[{"name":"大鹿, 周佐"}]},{"nameIdentifiers":[{"nameIdentifier":"11717","nameIdentifierScheme":"WEKO"}],"names":[{"name":"奈良岡, 琢哉"}]},{"nameIdentifiers":[{"nameIdentifier":"11718","nameIdentifierScheme":"WEKO"}],"names":[{"name":"藤, 哲"}]}]},"item_5_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_5_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_5_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"494.7","subitem_subject_scheme":"NDC"}]},"item_5_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"外科系臨床医学","subitem_subject_scheme":"Other"}]},"item_5_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Orthopedic Surgery, Towada City Hospital"},{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"弘前大学大学院医学研究科整形外科"},{"subitem_text_value":"弘前大学大学院医学研究科整形外科"},{"subitem_text_value":"十和田市立中央病院整形外科"},{"subitem_text_value":"弘前大学大学院医学研究科整形外科"},{"subitem_text_value":"弘前大学大学院医学研究科整形外科"}]},"item_5_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uesato, Ryoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishibashi, Yasuyuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ohshika, Shusa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naraoka, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toh, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"AN00211444_59_98.pdf","filesize":[{"value":"882.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00211444_59_98.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3610/files/AN00211444_59_98.pdf"},"version_id":"2a685b95-83ca-45b4-aec2-fcf9bd1356c9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mesenchymal stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"proteoglycan","subitem_subject_scheme":"Other"},{"subitem_subject":"chondrogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"間葉系幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオグリカン","subitem_subject_scheme":"Other"},{"subitem_subject":"軟骨分化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of Exogenous Proteoglycans on Chondrogenesis of Bone Marrow Mesenchymal Stem Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Exogenous Proteoglycans on Chondrogenesis of Bone Marrow Mesenchymal Stem Cells"}]},"item_type_id":"5","owner":"3","path":["642"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-08-20"},"publish_date":"2008-08-20","publish_status":"0","recid":"3610","relation_version_is_last":true,"title":["Effect of Exogenous Proteoglycans on Chondrogenesis of Bone Marrow Mesenchymal Stem Cells"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:24:59.376583+00:00"}