{"created":"2023-05-15T09:02:45.293381+00:00","id":3622,"links":{},"metadata":{"_buckets":{"deposit":"b5b62fec-5f34-4b3e-837d-7b10caf097a9"},"_deposit":{"created_by":3,"id":"3622","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3622"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003622","sets":["557:561:643"]},"author_link":["11817"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"S57","bibliographicPageStart":"S52","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recent studies show the involvement of cytoplasmic RNA helicase family, RIG-I, MDA5 and LGP2 in\nantiviral innate immune responses. RIG-I and MDA5 are primarily responsible for the detection of viral infection\nand triggering activation cascade for type I interferon genes in many cell types. RIG-I consists of N-terminal\nCAspase Recruitment Domain (CARD) and a domain with signatures of DExD/H box helicase (helicase domain).\nFunctional analyses revealed that the helicase domain detects viral RNA and CARD triggers the activation of\ndownstream signaling cascade, including activation of transcription factors, NF-κB, IRF-3 and IRF-7. RIG-I binds\nto double stranded (ds)RNA, however it does not simply function as a binding receptor for dsRNA, since RIG-I\nwith disrupted ATP binding site is incapable of signaling. A model is proposed that in the absence of dsRNA,\nRIG-I forms “closed” conformation and upon binding to dsRNA, it conforms into “open” structure exposing CARD.\nWe produced recombinant RIG-I protein using Baculo virus system and purified it to homogeneity. Biochemical\nproperties, including dsRNA binding activity, ATPase activity and helicase activity, of recombinant RIG-I were\ninvestigated. The results suggested that RIG-I requires certain structure of ligand RNA that is specifi c to viral (or\nnon-self) origin. Furthermore, we found evidence that RIG-I conforms a certain structure upon binding to dsRNA in\nthe presence of ATP. These results were consistent with the above model for activation of RIG-I. Furthermore, we\nobserved that RIG-I forms oligomers in virus-infected cells and artifi cial oligomerization of RIG-I CARD mimics virusinduced\nsignaling, resulting in the activation of interferon and other cytokine genes. These results highlight how viral\nreplication in cytoplasm is detected by RIG-I helicase and switch on signal cascades for initial antiviral responses.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 59(Suppl.), 2007, p.S52-S57","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.8","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"基礎医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Laboratory of Molecular Genetics, Institute for Virus Research, Kyoto University"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"11817","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_59_S52.pdf","filesize":[{"value":"508.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_59_S52.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3622/files/HirosakiMedJ_59_S52.pdf"},"version_id":"002a8d4f-8a0a-42d4-a4f5-b0c9318adb5d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sensors for replicating viruses and innate immunity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sensors for replicating viruses and innate immunity"}]},"item_type_id":"3","owner":"3","path":["643"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-18"},"publish_date":"2009-09-18","publish_status":"0","recid":"3622","relation_version_is_last":true,"title":["Sensors for replicating viruses and innate immunity"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T06:42:49.575053+00:00"}