{"created":"2023-05-15T09:02:46.209354+00:00","id":3637,"links":{},"metadata":{"_buckets":{"deposit":"e912b941-bf5c-4347-a91d-54b663685c7c"},"_deposit":{"created_by":3,"id":"3637","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3637"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003637","sets":["557:561:643"]},"author_link":["11888","11885","11602","11889","11891","11892","11601","11894","11895","11896","11600","11606","11610","11887","11604","11890","11893"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"S166","bibliographicPageStart":"S162","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" Bacillus Calmette-Guerin（ BCG） has been widely accepted as an eff ective treatment for CIS and superfi cial  carcinoma of the bladder. However, it has considerable side eff ects and toxicity. We thought if eff ective bacterial cell  wall elements as substitutes for live bacteria were identifi ed, useful treatment with lower toxicity while maintaining  strong anti-tumor eff ects might be possible. For these reasons we generated a nano-particulated BCG complex, which  does not contain live bacteria. Here, we present its direct in vitro anti-tumor eff ect on bladder cancer cell lines. Tokyo  172 BCG sub-strain was disrupted by French press with monitoring the particle distribution by the particle analyzer.  After removing the not-disrupted bacteria by centrifuge at 6,800×g, supernatant was centrifuged at 18,000×g. Then the  supernatant（ Sup） and the precipitate（ CW） were lyophilyzed to obtain nano-particulated BCG complex. Bladder cancer  cell lines, J82 and KK47, were co-cultured with BCG, Sup, CW or Sup+CW（ mix） for 5 days, then viable cell numbers  were counted. In J82 cells, when separately added to the culture medium, both CW and Sup reduced cell number to  about 70% of control cells. While they were mixed together, they reduced cell number equally compared with BCG; 59.2% （mix） vs 60.2%（ BCG） in J82 cells, 67.3% vs 68.8% in KK47 cells. These preliminary in vitro experiments demonstrated  the identical direct anti-tumor eff ect of nano-particulated BCG to that of live BCG. In vivo tumor assays are warranted  for clinical application of nano-particulated BCG. ","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 59(Suppl.), 2007, p.S162-S166","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"493.8","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"内科系臨床医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Japan BCG Central Laboratory"},{"subitem_text_value":"Japan BCG Central Laboratory"},{"subitem_text_value":"Japan BCG Central Laboratory"},{"subitem_text_value":"Department of Urology, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mori, Kazuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshikawa, Kazuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saitoh, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudoh, Seiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imai, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishimura, Hirofumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hatakeyama, Shingo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hagisawa, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwabuchi, Ikuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoneyama, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koie, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamato, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokomizo, Hidehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naka, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yano, Ikuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Chikara"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_59_S162.pdf","filesize":[{"value":"241.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_59_S162.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3637/files/HirosakiMedJ_59_S162.pdf"},"version_id":"fe721c6a-cfc3-4fe2-b15c-28b292141fa5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"nano-particulated BCG","subitem_subject_scheme":"Other"},{"subitem_subject":"direct anti-tumor effect","subitem_subject_scheme":"Other"},{"subitem_subject":"bladder cancer cell line","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line"}]},"item_type_id":"3","owner":"3","path":["643"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-18"},"publish_date":"2009-09-18","publish_status":"0","recid":"3637","relation_version_is_last":true,"title":["Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:00:20.512759+00:00"}