{"created":"2023-05-15T09:02:46.296427+00:00","id":3639,"links":{},"metadata":{"_buckets":{"deposit":"0cdc6d5c-0b9c-4d41-9436-a75867028129"},"_deposit":{"created_by":3,"id":"3639","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3639"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003639","sets":["557:561:643"]},"author_link":["11903","11868","11870","11899","11904","11290","11876","11902"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"S187","bibliographicPageStart":"S172","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The application of mechanical stress is known to be the unique method of analyses on bone metabolism\nand remodeling in vitro and in vivo. We have investigated on the effects of the application of cyclic tensile strain\n（CTS） on the syntheses of bone metabolic factors from the normal human osteoblasts in vitro. In this study, we found\nthat diff erent passage numbers aff ected synthesis of CTS-induced factors from osteoblasts in vitro. Osteoblasts were\nstimulated with the application of CTS for 3 days, 4 hrs a day. Then, it was found that the application of CTS increased\nnitric oxide（ NO） production, the expression of cyclooxygenase 2（ Cox-2） mRNA, and synthesis of osteopontin（ OPN）\nfrom osteoblasts during serial passage. However, CTS-induced osteoprotegerin（ OPG） synthesis from osteoblasts was\nshown to change at diff erent passage numbers. In short, increasing at the 3rd passage, CTS-induced OPG synthesis from\nosteoblasts decreased at the 5th passage. While, though soluble RANKL（ sRANKL） release by the application of CTS\ndecreased at 1st passage and did not change at the 5th passage, the expression of receptor activator of nuclear factor-κB\nligand（ RANKL） mRNA increased in osteoblasts at the 5th passage, decreasing in those at the 3rd passage. The increase\non OPN synthesis the expression of Cox-2 mRNA, and NO production from osteoblasts by the application of CTS was\nnot aff ected during serial passage. Analyzing on the activity of the p38 mitogen-activated protein kinase（ p38 MAPK）\nin osteoblasts with or without the application of CTS, the p38 MAPK activity in osteoblasts at the 3rd passage inhibited\nin compared with that at the 3rd passage. From these results, we should use osteoblasts at the 1st passage when\nanalyzing on CTS-induced OPG synthesis with normal human osteoblasts. It is suggested that the regulation of CTSinduced\nOPG synthesis might be aff ected by aging of osteoblasts.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 59(Suppl.), 2007, p.S172-S187","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"基礎医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"},{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"},{"subitem_text_value":"Pathology and Bioscience, National University Corporation, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"},{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"},{"subitem_text_value":"The Department of Oral Anatomy, Showa University School of Dentistry"},{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"},{"subitem_text_value":"The Department of Dentistry and Oral Surgery Hirosaki University Graduate Schoolof Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kusumi, Akinori"}],"nameIdentifiers":[{"nameIdentifier":"11899","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakaki, Hirotaka"}],"nameIdentifiers":[{"nameIdentifier":"11870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kusumi, Tomomi"}],"nameIdentifiers":[{"nameIdentifier":"11290","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"11902","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Kosei"}],"nameIdentifiers":[{"nameIdentifier":"11868","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"100000220","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/100000220_ja.html"}]},{"creatorNames":[{"creatorName":"Oda, Mitsuo"}],"nameIdentifiers":[{"nameIdentifier":"11903","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Satoh, Hisashi"}],"nameIdentifiers":[{"nameIdentifier":"11904","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Hiroto"}],"nameIdentifiers":[{"nameIdentifier":"11876","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_59_S172.pdf","filesize":[{"value":"872.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_59_S172.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3639/files/HirosakiMedJ_59_S172.pdf"},"version_id":"f7724ac9-d5f3-4afe-bd5a-35d0970706d2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro"}]},"item_type_id":"3","owner":"3","path":["643"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-18"},"publish_date":"2009-09-18","publish_status":"0","recid":"3639","relation_version_is_last":true,"title":["The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:01:17.712547+00:00"}