{"created":"2023-05-15T09:02:47.195070+00:00","id":3658,"links":{},"metadata":{"_buckets":{"deposit":"8db180ec-c392-406c-8100-d8c945a10a5b"},"_deposit":{"created_by":3,"id":"3658","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3658"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003658","sets":["557:561:644"]},"author_link":["12002","11997","12003","12007","12006","11998","12000","12001","11996","12008","12004","11995","11999","11861","12005"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ラットC6 グリオーマ細胞におけるケタミンとセボフルランの抗炎症作用関連遺伝子のスクリーニング"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ラット C6 グリオーマ サイボウ ニオケル ケタミン ト セボフルラン ノ コウエンショウ サヨウ カンレン イデンシ ノ スクリーニング"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1/2/3/4","bibliographicPageEnd":"35","bibliographicPageStart":"27","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, we have conducted cDNA microarray in C6, rat brain glioma cell line, to assess anti-infl ammatory eff ects of ketamine and sevofl urane in the central nervous system. The cultured C6 cells were treated with ketamine （0-100 μM） and sevofl urane （0 and 0.66 mM）. Total RNA was extracted from the cells and labeled with fl uorescent dye and then hybridized with microarray slide, containing 1936 genes. Quantitative analysis of each gene expression was confi rmed by real-time polymerase chain reaction （PCR）. Microarray analyses showed that ketamine downregulated the expression of 4 proinfl ammatory cytokine genes and upregulated that of 2 antiinfl ammatory cytokine genes. On the other hand, sevofl urane downregulated the expression of 2 proinfl ammatory cytokines but upregulated that of two other proinfl ammatory cytokines. Furthermore, sevofl urane failed to stimulate the expressions of anti-infl ammatory cytokines. Although patterns of cytokine expression in response to ketamine and sevofl urane were diff erent from each other described above, both anesthetics downregulated a key cytokine, interleukin（ IL）-1β remarkably in microarray analysis, which was confi rmed by real time PCR. These results suggest that both ketamine and sevofl urane show mainly anti-infl ammatory properties through the inhibition of IL-1β.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 60(1/2/3/4), 2009, p.27-35","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11999","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ニワ, ヒデトモ"}]},{"nameIdentifiers":[{"nameIdentifier":"12000","nameIdentifierScheme":"WEKO"}],"names":[{"name":"サカイ, テツヒロ"}]},{"nameIdentifiers":[{"nameIdentifier":"12001","nameIdentifierScheme":"WEKO"}],"names":[{"name":"フルカワ, ケンイチ"}]},{"nameIdentifiers":[{"nameIdentifier":"12002","nameIdentifierScheme":"WEKO"}],"names":[{"name":"カネマル, コウタ"}]},{"nameIdentifiers":[{"nameIdentifier":"12003","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヒロタ, カズヨシ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12004","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"100000249","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/100000249_ja.html"}],"names":[{"name":"丹羽, 英智"}]},{"nameIdentifiers":[{"nameIdentifier":"12005","nameIdentifierScheme":"WEKO"}],"names":[{"name":"坂井, 哲博"}]},{"nameIdentifiers":[{"nameIdentifier":"12006","nameIdentifierScheme":"WEKO"}],"names":[{"name":"古川, 賢一"}]},{"nameIdentifiers":[{"nameIdentifier":"12007","nameIdentifierScheme":"WEKO"}],"names":[{"name":"金丸, 幸太"}]},{"nameIdentifiers":[{"nameIdentifier":"12008","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"293","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/293_ja.html"}],"names":[{"name":"廣田, 和美"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.3","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"内科系臨床医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Anesthesiology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Anesthesiology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pharmacology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pharmacology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Anesthesiology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"弘前大学大学院医学研究科麻酔科学講座"},{"subitem_text_value":"弘前大学大学院医学研究科麻酔科学講座"},{"subitem_text_value":"弘前大学大学院医学研究科病態薬理学講座"},{"subitem_text_value":"弘前大学大学院医学研究科病態薬理学講座"},{"subitem_text_value":"弘前大学大学院医学研究科麻酔科学講座"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Niwa, Hidetomo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sakai, Tetsuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furukawa, Ken-Ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanemaru, Kota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirota, Kazuyoshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_60_1-4_27.pdf","filesize":[{"value":"260.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_60_1-4_27.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3658/files/HirosakiMedJ_60_1-4_27.pdf"},"version_id":"bbc18f44-3501-474c-b213-ac650ab93b65"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ketamine","subitem_subject_scheme":"Other"},{"subitem_subject":"Sevoflurane","subitem_subject_scheme":"Other"},{"subitem_subject":"Anti-inflammatory properties","subitem_subject_scheme":"Other"},{"subitem_subject":"Brain cells","subitem_subject_scheme":"Other"},{"subitem_subject":"ケタミン","subitem_subject_scheme":"Other"},{"subitem_subject":"セボフルラン","subitem_subject_scheme":"Other"},{"subitem_subject":"抗炎症作用","subitem_subject_scheme":"Other"},{"subitem_subject":"脳細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells"}]},"item_type_id":"3","owner":"3","path":["644"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-08"},"publish_date":"2009-04-08","publish_status":"0","recid":"3658","relation_version_is_last":true,"title":["A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T11:20:41.233560+00:00"}