{"created":"2023-05-15T09:02:47.662738+00:00","id":3668,"links":{},"metadata":{"_buckets":{"deposit":"ba77cd44-5901-4d87-9d48-9b0c42905501"},"_deposit":{"created_by":3,"id":"3668","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3668"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003668","sets":["557:561:645"]},"author_link":["11727","12125","12169","12137","11023","11737","11026","12170","12174","11029","11747","12131"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"悪性胸膜中皮腫細胞株に対するメシル酸ナファモスタット（FUT-175）の細胞増殖及び細胞浸潤抑制効果の検討"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"アクセイ キョウマク チュウヒシュ サイボウカブ ニ タイスル メシルサン ナファモスタット FUT-175 ノ サイボウ ゾウショク オヨビ サイボウ シンジュン ヨクセイ コウカ ノ ケントウ"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"25","bibliographicPageStart":"19","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" Nafamostat mesilate（ FUT-175）, a synthetic serine protease inhibitor, has been reported to have antitumouractivities toward solid tumours. The objective of this study was to characterize the biological activities of FUT-175 ina malignant mesothelioma cell line. We used MSTO-211H, a biphasic-type human malignant pleural mesothelioma cellline. The effect on cell growth was evaluated by 3（- 4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay.Secretion of urokinase-type plasminogen activator（ u-PA） and plasminogen activator inhibitor-1（ PAI-1） was analysedby enzyme-linked immunosorbent assay. The eff ects on relative mRNA expression levels were measured by reversetranscription polymerase chain reaction. The eff ect on cell invasiveness was evaluated by cell invasion assay. FUT-175at 10-5 M signifi cantly inhibited cell growth and cell invasiveness. Cell growth reduced to 47.0 ± 2.1% compared withthe control. The number of invasive cells also reduced to 16.0 ± 0.7 cells/hpf, while that of control cells was 41.4 ± 8.0cells /hpf. U-PA and PAI-1 secreted from the cells were also reduced by FUT-175 in a dose-dependent manner. Theseresults suggest that FUT-175 has the potential to act as a therapeutic agent against local growth and invasion, andfunctions by reducing PAI-1 and u-PA production of the human malignant mesothelioma（195 words）","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 61(1), 2010, p.19-25","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12170","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ストウ, タケミチ"}]},{"nameIdentifiers":[{"nameIdentifier":"11026","nameIdentifierScheme":"WEKO"}],"names":[{"name":"フクダ, イクオ"}]},{"nameIdentifiers":[{"nameIdentifier":"12131","nameIdentifierScheme":"WEKO"}],"names":[{"name":"キムラ, ダイスケ"}]},{"nameIdentifiers":[{"nameIdentifier":"11737","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ツシマ, タカオ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12174","nameIdentifierScheme":"WEKO"}],"names":[{"name":"須藤, 武道"}]},{"nameIdentifiers":[{"nameIdentifier":"11023","nameIdentifierScheme":"WEKO"}],"names":[{"name":"福田, 幾夫"}]},{"nameIdentifiers":[{"nameIdentifier":"12137","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"493","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/493_ja.html"}],"names":[{"name":"木村, 大輔"}]},{"nameIdentifiers":[{"nameIdentifier":"11727","nameIdentifierScheme":"WEKO"}],"names":[{"name":"対馬, 敬夫"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学出版会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"494.5","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"外科系臨床医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sutoh, Takemichi"}],"nameIdentifiers":[{"nameIdentifier":"12169","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukuda, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"11029","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"12125","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"493","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/493_ja.html"}]},{"creatorNames":[{"creatorName":"Tsushima, Takao"}],"nameIdentifiers":[{"nameIdentifier":"11747","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_61(1)_19.pdf","filesize":[{"value":"224.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_61(1)_19.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3668/files/HirosakiMedJ_61(1)_19.pdf"},"version_id":"713c97bf-edf4-4a60-93e9-b35b7257e865"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"nafamostat mesilate","subitem_subject_scheme":"Other"},{"subitem_subject":"malignant pleural mesothelioma","subitem_subject_scheme":"Other"},{"subitem_subject":"urokinase-type plasminogen activator","subitem_subject_scheme":"Other"},{"subitem_subject":"plasminogen activator inhibitor-1","subitem_subject_scheme":"Other"},{"subitem_subject":"メシル酸ナファモスタット","subitem_subject_scheme":"Other"},{"subitem_subject":"悪性胸膜中皮腫","subitem_subject_scheme":"Other"},{"subitem_subject":"ウロキナーゼ型プラスミノーゲン","subitem_subject_scheme":"Other"},{"subitem_subject":"活性化因子","subitem_subject_scheme":"Other"},{"subitem_subject":"プラスミノーゲン活性化因子インヒビター1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nafamostat Mesilate（ FUT-175） Inhibits Cell Growth and Invasion of Malignant Pleural Mesothelioma Cell Line, MSTO-211H","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nafamostat Mesilate（ FUT-175） Inhibits Cell Growth and Invasion of Malignant Pleural Mesothelioma Cell Line, MSTO-211H"}]},"item_type_id":"3","owner":"3","path":["645"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-29"},"publish_date":"2010-03-29","publish_status":"0","recid":"3668","relation_version_is_last":true,"title":["Nafamostat Mesilate（ FUT-175） Inhibits Cell Growth and Invasion of Malignant Pleural Mesothelioma Cell Line, MSTO-211H"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:17:19.566975+00:00"}