@article{oai:hirosaki.repo.nii.ac.jp:00003669,
 author = {Nodagashira, Tatsuya and Odagiri, Hiroki and Ikenaga, Shojiro-Kazunori and Maruyama, Masateru and Sato, Toshiyuki and Hakamada, Kenichi},
 issue = {1},
 journal = {弘前医学},
 month = {Mar},
 note = {Tissue-specifi c promoter has been used for cancer-specifi c suicide gene therapy, but its transcriptional
activity is relatively low. For more effi cient gene therapy of HER2-expressing tumor, a double adenovirus infection
system was established, in which a ‘regulator’ vector carried Cre gene under the control of HER2 promoter and ‘target’
vectors carried target genes activated by Cre. We constructed a Cre recombinase expression vector, AxHER2Cre, for
the ‘regulator’ vector. By the combination of this vector and AxCALNLZ, β-D-galactosidase was induced in 90% and
70% of MKN7 and MDA-MB-453, HER2‒overexpressing cell lines, but only about 20% and 10% of MKN28 and MCF7,
low HER2-expressing cell lines. By the quantifi cation analysis, the β-galactosidase activities induced by this system
were comparable to those by the combination of AxCANCre and AxCALNLZ. These results indicated that Cre/
loxP system under the regulation of HER2 promoter could induce effi cient gene expression, maintaining the HER2-
expression specifi city. Breast cancer with HER2 overexpression is treated with trastuzumab. However, refractory
or resitance of HER2 positive breast cancer against trastuzumab becomes a severe clinical problem, recently. This
system seemed to be another therapeutic option., 弘前医学. 61(1), 2010, p.26-34},
 pages = {26--34},
 title = {Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System},
 volume = {61},
 year = {2010}
}