{"created":"2023-05-15T09:02:47.727087+00:00","id":3669,"links":{},"metadata":{"_buckets":{"deposit":"a765c7a3-9dfc-46a3-9850-4f1180090084"},"_deposit":{"created_by":3,"id":"3669","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3669"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003669","sets":["557:561:645"]},"author_link":["12191","12189","12180","12178","11302","12185","11307","12182","12190","12186","12193","12187","11312","12184","12181","12183","12192","12179"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Cre/loxP system を用いたHER2/neu 発現細胞に対する特異的かつ効率的遺伝子導入システムの開発"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"Cre/loxP system オ モチイタ HER2/neu ハツゲン サイボウ ニ タイスル トクイテキ カツ コウリツテキ イデンシ ドウニュウ システム ノ カイハツ"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"34","bibliographicPageStart":"26","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"　Tissue-specifi c promoter has been used for cancer-specifi c suicide gene therapy, but its transcriptional\nactivity is relatively low. For more effi cient gene therapy of HER2-expressing tumor, a double adenovirus infection\nsystem was established, in which a ‘regulator’ vector carried Cre gene under the control of HER2 promoter and ‘target’\nvectors carried target genes activated by Cre. We constructed a Cre recombinase expression vector, AxHER2Cre, for\nthe ‘regulator’ vector. By the combination of this vector and AxCALNLZ, β-D-galactosidase was induced in 90% and\n70% of MKN7 and MDA-MB-453, HER2‒overexpressing cell lines, but only about 20% and 10% of MKN28 and MCF7,\nlow HER2-expressing cell lines. By the quantifi cation analysis, the β-galactosidase activities induced by this system\nwere comparable to those by the combination of AxCANCre and AxCALNLZ. These results indicated that Cre/\nloxP system under the regulation of HER2 promoter could induce effi cient gene expression, maintaining the HER2-\nexpression specifi city. Breast cancer with HER2 overexpression is treated with trastuzumab. However, refractory\nor resitance of HER2 positive breast cancer against trastuzumab becomes a severe clinical problem, recently. This\nsystem seemed to be another therapeutic option.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 61(1), 2010, p.26-34","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12183","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ノダガシラ, タツヤ"}]},{"nameIdentifiers":[{"nameIdentifier":"12184","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オダギリ, ヒロキ"}]},{"nameIdentifiers":[{"nameIdentifier":"12185","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イケナガ, ショウジロウカズノリ"}]},{"nameIdentifiers":[{"nameIdentifier":"12186","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マルヤマ, マサテル"}]},{"nameIdentifiers":[{"nameIdentifier":"12187","nameIdentifierScheme":"WEKO"}],"names":[{"name":"サトウ, トシユキ"}]},{"nameIdentifiers":[{"nameIdentifier":"11312","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハカマダ, ケンイチ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12189","nameIdentifierScheme":"WEKO"}],"names":[{"name":"野田頭, 達也"}]},{"nameIdentifiers":[{"nameIdentifier":"12190","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小田桐, 弘毅"}]},{"nameIdentifiers":[{"nameIdentifier":"12191","nameIdentifierScheme":"WEKO"}],"names":[{"name":"池永, 照史郎一期"}]},{"nameIdentifiers":[{"nameIdentifier":"12192","nameIdentifierScheme":"WEKO"}],"names":[{"name":"丸山, 将輝"}]},{"nameIdentifiers":[{"nameIdentifier":"12193","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 利行"}]},{"nameIdentifiers":[{"nameIdentifier":"11307","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"257","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/257_ja.html"}],"names":[{"name":"袴田, 健一"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学出版会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491.6","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"基礎医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nodagashira, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"12178","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Odagiri, Hiroki"}],"nameIdentifiers":[{"nameIdentifier":"12179","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikenaga, Shojiro-Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"12180","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maruyama, Masateru"}],"nameIdentifiers":[{"nameIdentifier":"12181","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"12182","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hakamada, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"11302","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"257","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/257_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_61(1)_26.pdf","filesize":[{"value":"340.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_61(1)_26.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3669/files/HirosakiMedJ_61(1)_26.pdf"},"version_id":"2240ccdb-255e-4ce3-9763-c57b5444a25d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HER2/c-erbB-2","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant adenovirus vector","subitem_subject_scheme":"Other"},{"subitem_subject":"Cre/loxP system","subitem_subject_scheme":"Other"},{"subitem_subject":"cancer specifi c promoter","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子/タンパク","subitem_subject_scheme":"Other"},{"subitem_subject":"組換えアデノウイルスベクター","subitem_subject_scheme":"Other"},{"subitem_subject":"Cre/loxP システム","subitem_subject_scheme":"Other"},{"subitem_subject":"癌特異的プロモーター","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System"}]},"item_type_id":"3","owner":"3","path":["645"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-03-29"},"publish_date":"2010-03-29","publish_status":"0","recid":"3669","relation_version_is_last":true,"title":["Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T09:43:12.331001+00:00"}