@article{oai:hirosaki.repo.nii.ac.jp:00003670,
 author = {Sato, Hideaki and Sato, Masashi and Chiba, Makie and Ito, Kyoko and Ito, Koichi},
 issue = {1},
 journal = {弘前医学},
 month = {Mar},
 note = {The ability of murine allogeneic umbilical cord blood cells （UCBCs） to reconstitute the immune system
was investigated. UCBCs obtained from fetuses of C57BL/6 （B6; H-2b） mice, which were transgenic for green
fluorescent protein （GFP）, were transplanted into RAG2 （-/-） BALB/c mice （H-2d）. After transplantation, flow
cytometric analysis revealed successful reconstitution of phenotypically mature GFP-positive immune cells of donor
origin, including T cells, B cells, monocytes, and granulocytes in the peripheral blood of the recipient mice. Analysis
of functional maturation of lymphocytes revealed that 2,4,6-trinitrophenyl-keyhole limpet hemocyanin （TNP-KLH）
-immunized UCBC-transplanted recipients produced both TNP-specific IgM and IgG antibodies. These results
indicated that the recipient mice were capable of mounting antibody responses to T-dependent antigens; further, Ig
class switching from IgM to IgG confi rmed that both B cells and CD4+ helper T cells derived from allogeneic UCBCs
were immunologically competent. Furthermore, mice transplanted with allogeneic UCBCs accepted skin grafts from
both B6 and BALB/c mice. However, these chimeric mice completely rejected skin grafts from third party C3H/
HeJ （H-2k） mice, indicating the presence of functional CD8+ killer T cells as well as CD4+ helper T cells. In terms
of potential clinical application, our results indicate that allogeneic UCBC transplantation can enable recovery of the
normal immune system in recipients., 弘前医学. 61(1), 2010, p.35-45},
 pages = {35--45},
 title = {Reconstitution of the Immune System after Murine Allogeneic Umbilical Cord Blood Transplantation},
 volume = {61},
 year = {2010}
}