@article{oai:hirosaki.repo.nii.ac.jp:00003674,
 author = {Abe, Kazuhiro and Higuchi, Tsuyoshi and Mizunuma, Hideki and Kakizaki, Ikuko and Endo, Masahiko and Suto, Shinichiro},
 issue = {2-4},
 journal = {弘前医学},
 month = {Jan},
 note = {The aim of study is to analyze functional sugar chains, glycosaminoglycans （GAGs） in urine from
postmenopausal women with osteoporosis. A 24-hour urine was collected from 15 osteoporotic women and normal
women. All women were postmenopausal and aged at 60 to 70 years old. Diagnosis of osteoporosis was based on
the diagnosis criteria proposed by the Japan Osteoporosis Society after measurement of lumbar spine bone mineral
density by dual energy X-ray absorptiometry. The urine was concentrated by dialysis and evaporation, then
precipitated with ethanol. The precipitate was then reconstituted into NaCl solution, precipitated again by adding 10% cetylpyridinium chloride and the precipitation was used as complex GAGs fraction. The yield of complex fraction
was significantly higher in osteoporotic subjects than that in normal control. Then, the complex GAGs fraction was
analyzed by cellulose acetate membrane electrophoresis and high performance liquid chromatography （HPLC）,
respectively. Cellulose acetate membrane electrophoresis has revealed that GAGs in urine contained partially
desulfated chondroitin sulfate. However, further analysis by HPLC has shown that the excretion of desulfated GAGs
was rather less in osteoporotic women, indicating that urinary GAGs fraction from osteoporotic women contains
unknown acidic polysaccharides as well as GAGs., 弘前医学. 61(2-4), 2011, p.97-103},
 pages = {97--103},
 title = {ANALYSIS OF FUNCTIONAL SUGAR CHAINS OF PROTEOGLYCANS IN THE URINE FROM WOMEN WITH OSTEOPOROSIS},
 volume = {61},
 year = {2011}
}