@article{oai:hirosaki.repo.nii.ac.jp:00003685,
 author = {Yamada, Katsuya and Yamamoto, Toshihiro and Watanabe, Seiji and Nishiuchi, Yuji and Teshima, Tadashi and Matsuoka, Hideaki and Suga, Sechiko},
 issue = {Supplement},
 journal = {弘前医学},
 month = {Jul},
 note = {Glucose transport activity in mammalian cells has been monitored by radiolabeled tracers such as [14C] 2-deoxy-D-glucose. However, due to their limited spatial and temporal resolution, measuring glucose uptake in single, living cells was diffi cult. We have developed a fl uorescent D-glucose derivative, 2-[N（- 7-nitrobenz-2-oxa-1,3-diazol-4-yl）amino]-2-deoxy-D-glucose （2-NBDG）, that allows a more sensitive measurement of glucose uptake in live cells. Indeed, the uptake of 2-NBDG takes place through glucose transporters （GLUTs） in a concentration-, time-, and temperature-dependent manner. Kinetic analysis revealed that apparent Km values for the uptake were similar to those reported by D-glucose and the nonmetabolizable glucose analogue, 3-O-methyl-D-glucose, found in pancreatic islet cells. This method can be combined with Ca2+ imaging and subsequent immunocytochemical identifi cation of cells. So far, 2-NBDG has been used for monitoring glucose uptake into a wide variety of mammalian cells including astrocytes and neurons. However, it has been pointed out that 2-NBDG method requires very accurate procedures, since the fl uorescence intensity is an arbitrary measure. In addition, temporal changes in health of cells in question should be verifi ed throughout the experiment by an independent way such as using patch clamp. To overcome the diffi culty, we synthesized transporter- unrecognizable glucose analogues as control for 2-NBDG uptake. These include 2-[N（- 7-Nitrobenz-2-oxa-1,3-diazol-4-yl）amino]-2-deoxy-L-glucose [2-NBDLG], the antipode of 2-NBDG. A combined use of transporter-recognizable（ D-isomer） and unrecognizable（ L-isomer） fl uorescent analogues allows us to investigate not only net stereospecifi c transport of glucose in live cells but also ligand-transporter interactions and non-transportermediated glucose movement., 弘前医学. 61(Suppl.), 2010, p.S23-S25},
 pages = {S23--S25},
 title = {＜Symposium I＞A new imaging method of glucose transport in live cells},
 volume = {61},
 year = {2010}
}