{"created":"2023-05-15T09:02:48.621949+00:00","id":3688,"links":{},"metadata":{"_buckets":{"deposit":"6fda35db-21fc-4b7d-a4d0-5ee44813da51"},"_deposit":{"created_by":3,"id":"3688","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3688"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003688","sets":["557:561:647"]},"author_link":["12409","12406","12410","12411","20589","12423","11451","12408","11458","12415","20590","12420","12421","20591","20594","11452","12407","20593","11459","20588","12416","20592","12417","12412"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"S52","bibliographicPageStart":"S43","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The circadian rhythms in mammals are regulated by a pacemaker located in the suprachiasmatic nucleus of the hypothalamus. Five clock-gene families, i.e. Clock, Bmal, Per, Cry and Dec, have been found to be involved in a transcription-translation feedback loop that generates the circadian rhythm at the intracellular level. In this study, we examined functional analysis of the Dec gene. DEC1 and DEC2 are basic-helix-loop-helix （bHLH） transcription factors, involved in cellular diff erentiation, responses to hypoxia, and circadian rhythms. We recently showed that the expression of DEC1 and DEC2 was upregulated by hypoxia, however, the functions of these two factors under hypoxic conditions have not been elucidated in detail. It is well established that the expression of vascular endothelial growth factor （VEGF） is upregulated by hypoxia, and the expression of VEGF in response to hypoxia depends on transcriptional activation by a heterodimer comprising hypoxia-inducible factor 1 α （HIF-1α） and arylhydrocarbon receptor nuclear translocator 1 （ARNT1）. In the present study, we showed that DEC2, but not DEC1, suppressed VEGF gene expression under hypoxic conditions. DEC2 protein was co-immunoprecipitated with HIF-1α but not with ARNT1. The binding of HIF-1α to the hypoxia response element（ HRE） in the VEGF promoter was decreased by DEC2 overexpression, and increased by DEC2 knockdown. We also showed that the circadian expression of VEGF showed a reciprocal pattern to that of DEC2 in cartilage. DEC2 had a circadian oscillation in implanted Sarcoma 180 cells. We conclude that DEC2 negatively regulates VEGF expression and plays an important role in the pathological conditions in which VEGF is involved.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 61(Suppl.), 2010, p.S43-S52","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12420","nameIdentifierScheme":"WEKO"}],"names":[{"name":"キジマ, ヒロシ"}]},{"nameIdentifiers":[{"nameIdentifier":"12421","nameIdentifierScheme":"WEKO"}],"names":[{"name":"サトウ, フユキ"}]},{"nameIdentifiers":[{"nameIdentifier":"20588","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ウジャール, クマール　バイアル"}]},{"nameIdentifiers":[{"nameIdentifier":"20589","nameIdentifierScheme":"WEKO"}],"names":[{"name":"カワモト, タケシ"}]},{"nameIdentifiers":[{"nameIdentifier":"20590","nameIdentifierScheme":"WEKO"}],"names":[{"name":"フジモト, カツミ"}]},{"nameIdentifiers":[{"nameIdentifier":"11451","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イマイズミ, タダアツ"}]},{"nameIdentifiers":[{"nameIdentifier":"20591","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イマナカ, タダノブ"}]},{"nameIdentifiers":[{"nameIdentifier":"12423","nameIdentifierScheme":"WEKO"}],"names":[{"name":"コンドウ, ジュン"}]},{"nameIdentifiers":[{"nameIdentifier":"20592","nameIdentifierScheme":"WEKO"}],"names":[{"name":"コヤナギ, サトル"}]},{"nameIdentifiers":[{"nameIdentifier":"20593","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ノシロ, ミツヒデ"}]},{"nameIdentifiers":[{"nameIdentifier":"11452","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヨシダ, ヒデミ"}]},{"nameIdentifiers":[{"nameIdentifier":"20594","nameIdentifierScheme":"WEKO"}],"names":[{"name":"カトウ, ユキオ"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Pathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College"},{"subitem_text_value":"Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College"},{"subitem_text_value":"Department of Oral Maxillofacial Diagnostic Science, Division of Pathology & High-Tech Research Center, Kanagawa Dental College"},{"subitem_text_value":"Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of the Biology and Medicine of the Stem Cell, Hiroshima University Graduate School of Medical Science"},{"subitem_text_value":"Department of Pathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Medico-Pharmaceutical Sciences, Kyushu University"},{"subitem_text_value":"Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences"},{"subitem_text_value":"Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kijima, Hiroshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sato, Fuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Bhawal, Ujjal Kumar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawamoto, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimoto, Katsumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imaizumi, Tadaatsu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Imanaka, Tadanobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koyanagi, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Noshiro, Mitsuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Hidemi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Yukio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_61_S43.pdf","filesize":[{"value":"252.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_61_S43.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3688/files/HirosakiMedJ_61_S43.pdf"},"version_id":"cecc0bb9-7b8d-4494-9cb8-848222ef96ba"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"transcription factor","subitem_subject_scheme":"Other"},{"subitem_subject":"vascular endothelial growth factor (VEGF)","subitem_subject_scheme":"Other"},{"subitem_subject":"hypoxia","subitem_subject_scheme":"Other"},{"subitem_subject":"hypoxia-inducible factor-1α(HIF-1α)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"＜Symposium I＞Vascular endothelial growth factor（ VEGF） expression is negatively regulated by basic-helix-loop-helix（ bHLH）transcription factor DEC2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"＜Symposium I＞Vascular endothelial growth factor（ VEGF） expression is negatively regulated by basic-helix-loop-helix（ bHLH）transcription factor DEC2"}]},"item_type_id":"3","owner":"3","path":["647"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-08-16"},"publish_date":"2010-08-16","publish_status":"0","recid":"3688","relation_version_is_last":true,"title":["＜Symposium I＞Vascular endothelial growth factor（ VEGF） expression is negatively regulated by basic-helix-loop-helix（ bHLH）transcription factor DEC2"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T09:56:56.518174+00:00"}