{"created":"2023-05-15T09:02:48.946182+00:00","id":3693,"links":{},"metadata":{"_buckets":{"deposit":"58a41e93-fb35-44cc-861d-4a1ff14bf61f"},"_deposit":{"created_by":3,"id":"3693","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3693"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003693","sets":["557:561:647"]},"author_link":["9403","11869","9404","9399","9400","12447"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"S96","bibliographicPageStart":"S89","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"NEDD8 ultimate buster-1 （NUB1） is a potent down-regulator of the ubiquitin-like protein NEDD8, because it directly interacts with NEDD8 and targets it and its conjugates to the 26S proteasome for proteolytic degradation. Recently, we found that NUB1 physically interacts with synphilin-1 through its NEDD8-binding site, implying that NUB1 also targets synphilin-1 to the 26S proteasome for proteolytic degradation. Synphilin-1 is an α-synuclein-interacting protein and is a major component of inclusion bodies found in the brains of patients with neurodegenerative α-synucleinopathies, including Parkinson’s disease. In our recent studies, we immunostained sections of brains from patients with Parkinson’s disease and other α-synucleinopathies and demonstrated that NUB1, as well as synphilin-1, accumulates in the inclusion bodies. To defi ne the role of NUB1 in the formation of these inclusion bodies, we performed a co-transfection assay using cultured HEK293 cells. This assay showed that NUB1 suppresses the formation of synphilin-1-positive inclusions. Further biochemical assays revealed that NUB1 overexpression leads to the proteasomal degradation of synphilin-1. These results and our previous observations suggest that NUB1 indeed targets synphilin-1 to the proteasome for its effi cient degradation, which, because of the resultant reduction in synphilin-1, suppresses the formation of synphilin-1-positive inclusions. In addition to these basic science aspects, our fi ndings on NUB1 have two important bearings clinically. First, they suggest that NUB1 could serve as a neuropathological marker in patients with α-synucleinopathies because it is strongly accumulated with synphilin-1 in the inclusions of their brain cells. Second, they suggest that NUB1 could be a potential therapeutic target for α-synucleinopathies.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 61(Suppl.), 2010, p.S89-96","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"12447","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タンジ, クニカズ"}]},{"nameIdentifiers":[{"nameIdentifier":"9400","nameIdentifierScheme":"WEKO"}],"names":[{"name":"モリ, フミアキ"}]},{"nameIdentifiers":[{"nameIdentifier":"9399","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ワカバヤシ, コウイチ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11869","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"314","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/314_ja.html"}],"names":[{"name":"Tanji, Kunikazu"}]},{"nameIdentifiers":[{"nameIdentifier":"9404","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Mori, Fumiaki"}]},{"nameIdentifiers":[{"nameIdentifier":"9403","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Wakabayashi, Koichi"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Neuropathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Neuropathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Department of Neuropathology, Hirosaki University School of Medicine"},{"subitem_text_value":"Center for Molecular Chaperone, Medical College of Georgia"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanji, Kunikazu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Mori, Fumiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wakabayashi, Koichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_61_S89.pdf","filesize":[{"value":"193.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_61_S89.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3693/files/HirosakiMedJ_61_S89.pdf"},"version_id":"840f44ef-e0bb-46d9-8995-d174b111503a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Parkinson’s disease","subitem_subject_scheme":"Other"},{"subitem_subject":"NUB1","subitem_subject_scheme":"Other"},{"subitem_subject":"NEDD8","subitem_subject_scheme":"Other"},{"subitem_subject":"ubiquitin","subitem_subject_scheme":"Other"},{"subitem_subject":"proteasome","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"＜Symposium III＞Nubopathies : NUB1-related neurodegenerative diseases","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"＜Symposium III＞Nubopathies : NUB1-related neurodegenerative diseases"}]},"item_type_id":"3","owner":"3","path":["647"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-08-18"},"publish_date":"2010-08-18","publish_status":"0","recid":"3693","relation_version_is_last":true,"title":["＜Symposium III＞Nubopathies : NUB1-related neurodegenerative diseases"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T11:20:39.339916+00:00"}