@article{oai:hirosaki.repo.nii.ac.jp:00003713,
 author = {Narita, Koji and Hu, Dong-Liang and Nakane, Akio},
 issue = {Supplement},
 journal = {弘前医学},
 month = {Jul},
 note = {Clumping factor A （ClfA） is an adhesin of Staphylococcus aureus, and the residues 40-559 of ClfA （ClfA40-559） compose fi brinogen binding domain. It was reported that antibody to ClfA40-559 plays an important role in the protective eff ects. In this study, we investigated the role of IL-17 in the protective eff ects of an immunization with ClfA40-559 against S. aureus infection.
　Mice immunized or non-immunized with ClfA40-559 were challenged with S. aureus. Bacterial numbers in the organs and survival rates were evaluated. RAW264.7, DC2.4 and spleen cells of naïve and immunized mice were stimulated with ClfA40-559. Cytokine production and mRNA expression in the spleens and cells was determined by ELISA and/or RT-PCR.
　The survival rate was improved and the bacterial numbers in the organs were reduced in the mice immunized with ClfA40-559. ClfA40-559 induced IL-23p19 mRNA expression and IL-6 but not IL-12 production in DC2.4 and RAW264.7 cells. RORγt and IL-17A mRNA expression in the spleen cells of naïve mice was induced. In the spleen cells of immunized mice, ClfA40-559 induced the high production of IL-17 but not IFN-γ or IL-4. IL-17A, IL-6 and CXCL2 mRNA expression was also increased in the organs of immunized and challenged mice. These results suggest that an immunization with ClfA40-559 induces Th17, and that IL-17 contributes to host defense in early phase of S. aureus infection by promoting neutrophil recruitment., 弘前医学. 61(Suppl.), 2010, p.S238-S244},
 pages = {S238--S244},
 title = {＜Poster＞The role of interleukin-17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection},
 volume = {61},
 year = {2010}
}