@article{oai:hirosaki.repo.nii.ac.jp:00003801,
 author = {Yoshioka, Haruhiko and Hoshiai, Keita and Nakamura, Toshiya and Sato, Tatsusuke and Washiya, Kiyotada and Watanabe, Jun},
 issue = {1},
 journal = {弘前医学},
 month = {Apr},
 note = {Object: To investigate the usefulness of ultraviolet-microscopic spectroscopy （UV-MS） of unstained cells by liquid-based cytology （LBC） to objectively differentiate non-cancer from cancer cells. Study Design: Cultured cells were used as the sample cells: 100 non-cancer cells and 200 cancer cells. The sample measurement region was a 166.4-μm2 area in the nuclear region of sample cells. On UV-MS, data of 260, 280, 300, 320, and 340 nm were extracted from the transmittance spectrum of 260-350-nm ultraviolet （UV） wavelengths and analyzed. Result: At a 300-nm UV wavelength, transmittances in non-cancer and cancer cells were 79.7±5.0 and 64.1±5.0%, respectively, being signifi cantly lower in cancer cells （P<0.01）. On discriminant analysis, a linear discriminant function: Z=0.61 x transmittance（ 300 nm） - 44.02, was obtained. When the function value is positive and negative, the cell is judged as a cancer or non-cancer cell, respectively. The accuracy of this linear discriminant function was 96.3%, sensitivity was 98.0%, specifi city was 93.0%. Conclusions: UV-MS on unstained cells by LBC yields an objective value to discriminate non-cancer from cancer cells. Since LBC is a sample processing procedure which will be increasingly used in the future, we are planning to further investigate its applicability to clinical specimens based on this study., 弘前医学. 65, 2014, p.82-94},
 pages = {82--94},
 title = {Usefulness of Ultraviolet-Microscopic Spectroscopy on Unstained Cells by Liquid-Based Cytology for Objective Differentiation between Non-Cancer from Cancer Cells},
 volume = {65},
 year = {2014}
}