{"created":"2023-05-15T09:02:57.484380+00:00","id":3840,"links":{},"metadata":{"_buckets":{"deposit":"87032a64-326c-40cf-8741-fa68e8a9db8e"},"_deposit":{"created_by":3,"id":"3840","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3840"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003840","sets":["557:561:657"]},"author_link":["16164","14225","11977","15985","11875","16161","11976","11980","14527"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-04-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"27","bibliographicPageStart":"22","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: We reported that enhanced phospholipase C （PLC）-δ1, which was detected in patients with coronary spasm, caused coronary spasm in mice. We investigated the role of protein kinase C（ PKC） in acetylcholine （ACh）-induced Ca2+ influx under enhanced PLC-δ1 using human embryonic kidney（ HEK）-293 cells. Methods and Results: Intracellular free Ca2+ concentration （[Ca2+]i） was measured by fura-2, and Ca2+ influx was evaluated by the increase in [Ca2+]i after addition of extracellular Ca2+. ACh-induced Ca2+ influx（ nM） in HEK-293 cells was 21±2 in control HEK-293 cells, 52±6 in the cells with PLC-δ1 overexpression, and 75±9 in those with PLC-δ1 overexpression and PKC down-regulation （all p<0.05 among 3 groups）. Ca2+ influx under treatment with nifedipine at 10-5M was 2.9±0.1 times higher in the cells with PLC-δ1 overexpression and 5.6±0.2 times higher in those with PLC-δ1 overexpression and PKC down-regulation compared with the control cells（ all p<0.05 among 3 groups）. Conclusions: ACh-induced Ca2+ influx was enhanced by PLC-δ1 overexpression, but was attenuated by PKC activation. PKC plays an important role in Ca2+ influx under enhanced PLC-δ1 in a negative feedback fashion.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 66, 2015, p.22-27","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Cardiology, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Murakami, Kazuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Osanai, Tomohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Makoto"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kinjo, Takahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanno, Tomohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narita, Ikuyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Misato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomita, Hirofumi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Okumura, Ken"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_66(1)_22.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_66(1)_22.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3840/files/HirosakiMedJ_66(1)_22.pdf"},"version_id":"de7fe299-3937-4336-a4d7-0d6b39e1b4c0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Coronary spasm","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein kinase C","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase C-δ1","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium influx","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium influx","subitem_subject_scheme":"Other"},{"subitem_subject":"Acetylcholine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1"}]},"item_type_id":"3","owner":"3","path":["657"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-25"},"publish_date":"2015-05-25","publish_status":"0","recid":"3840","relation_version_is_last":true,"title":["Role of Protein Kinase C in Acetylcholine-Induced Ca2+ Influx under Enhanced Phospholipase C-δ1"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T09:42:37.521799+00:00"}