@article{oai:hirosaki.repo.nii.ac.jp:00003883,
 author = {Chong, Han and Ogata, Yoshiki and Niwa, Hidetoshi and Kushikata, Tetsuya and Watanabe, Hiroyuki and Imaizumi, Tadaatsu and Hirota, Kazuyoshi and Ono, Kyoichi and Ohba, Takayoshi and Murakami, Manabu},
 issue = {1},
 journal = {弘前医学},
 month = {Jul},
 note = {Transgenic mice experiments have become increasingly popular to research human inherited disease.
However, a number of Japanese researchers have difficulty with the selection of anesthesia, after the classification
of ketamine, probably the most used anesthesia, as a narcotic drug in 2006. Therefore, we compared the effects of
inhalation anesthesia （2% of isoflurane, sevoflurane and enflurane） and intraperitoneal pentobarbital anesthesia （50
mg/kg） on the electrocardiogram（ ECG） and blood oxygen saturation（ SPO2） of mice. With inhalation anesthesia, the
heart rate（ HR） and SPO2 were within an acceptable range. In contrast, the HR significantly decreased after initiation
of pentobarbital anesthesia, and gradually returned to a low rate. Importantly, pentobarbital anesthesia significantly
lowered SPO2, and heart rate variability analysis showed unstable beat-to-beat intervals during pentobarbital
anesthesia, suggesting that inhalation anesthesia is more suitable for evaluation of cardiorespiratory responses
than pentobarbital anesthesia. During anesthesia, propranolol, a ????-adrenergic blocker, significantly decreased heart
rate. Atropine, a parasympathetic blocker, also significantly increased heart rate. Our data suggest that inhalation 　anesthesia is suitable for cardiorespiratory analysis in mice., 弘前医学. 67, 2016, p.77-85},
 pages = {77--85},
 title = {The Cardiorespiratory Responses to Inhalation and Pentobarbital Anesthesia in the Mouse},
 volume = {67},
 year = {2016}
}