@article{oai:hirosaki.repo.nii.ac.jp:00003924,
 author = {Murakami, Manabu and Murakami, Agnieszka A and Ohba, Takayoshi},
 issue = {1},
 journal = {弘前医学},
 month = {Oct},
 note = {We analyzed transient and stable eukaryotic gene expression using internal ribosome entry site （IRES）
and green fluorescent protein （GFP）-S65T （IRES-GFP） expression systems. The IRES-containing vector enables
simple construction of a plasmid and easy detection of transfected cells, as well as an estimation of the levels of the
gene of interest. This system is useful for measuring changes in the intracellular calcium concentration using the
fluorescent calcium indicator Fura-2 AM, although subcellular localization of the protein of interest is not detectable.
Importantly, the IRES-GFP plasmid showed delayed expression and an approximate 40% reduction in fluorescence
intensity, which suggests that usage of IRES-containing expression system might cause under-estimation of desired
gene. Although this technique has some disadvantages, the IRES-containing vector is simple and straightforward for gene expression analysis, comparing with co-expression procedure of multiple plasmids., 弘前医学. 68, p.44-51. 2017},
 pages = {44--51},
 title = {A Comparative Study of The Internal Ribosome Entry Sitecontaining　Expression System},
 volume = {68},
 year = {2017}
}