{"created":"2023-05-15T09:03:02.395985+00:00","id":3924,"links":{},"metadata":{"_buckets":{"deposit":"07acdd3f-62b6-4fb2-8b5f-c4f9c6cb32ff"},"_deposit":{"created_by":3,"id":"3924","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3924"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003924","sets":["557:561:661"]},"author_link":["15023","14444","14438"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-10-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"44","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We analyzed transient and stable eukaryotic gene expression using internal ribosome entry site （IRES）\nand green fluorescent protein （GFP）-S65T （IRES-GFP） expression systems. The IRES-containing vector enables\nsimple construction of a plasmid and easy detection of transfected cells, as well as an estimation of the levels of the\ngene of interest. This system is useful for measuring changes in the intracellular calcium concentration using the\nfluorescent calcium indicator Fura-2 AM, although subcellular localization of the protein of interest is not detectable.\nImportantly, the IRES-GFP plasmid showed delayed expression and an approximate 40% reduction in fluorescence\nintensity, which suggests that usage of IRES-containing expression system might cause under-estimation of desired\ngene. Although this technique has some disadvantages, the IRES-containing vector is simple and straightforward for gene expression analysis, comparing with co-expression procedure of multiple plasmids.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 68, p.44-51. 2017","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pharmacology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Cellphysiology, Akita University School of Medicine"},{"subitem_text_value":"Department of Cellphysiology, Akita University School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Murakami, Manabu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Murakami, Agnieszka A"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohba, Takayoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_68(1)_44.pdf","filesize":[{"value":"230.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_68(1)_44.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3924/files/HirosakiMedJ_68(1)_44.pdf"},"version_id":"aea33a5d-65d4-47ff-80d7-428cd7f0a5c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"eukaryotic expression vector","subitem_subject_scheme":"Other"},{"subitem_subject":"bicistronic expression vector","subitem_subject_scheme":"Other"},{"subitem_subject":"green fluorescent protein","subitem_subject_scheme":"Other"},{"subitem_subject":"internal ribosome entry site.","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Comparative Study of The Internal Ribosome Entry Sitecontaining　Expression System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Comparative Study of The Internal Ribosome Entry Sitecontaining　Expression System"}]},"item_type_id":"3","owner":"3","path":["661"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-23"},"publish_date":"2017-10-23","publish_status":"0","recid":"3924","relation_version_is_last":true,"title":["A Comparative Study of The Internal Ribosome Entry Sitecontaining　Expression System"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T10:38:49.610253+00:00"}