{"created":"2023-05-15T09:03:02.465549+00:00","id":3925,"links":{},"metadata":{"_buckets":{"deposit":"74427380-6490-44bb-8797-41216f42035d"},"_deposit":{"created_by":3,"id":"3925","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3925"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00003925","sets":["557:561:661"]},"author_link":["14831","12108","11458","11997"],"control_number":"3925","item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-10-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"61","bibliographicPageStart":"52","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two sensitive enzymatic fluorometric assays have been developed for adenosine 3’,5’-cyclic monophosphate\n（cAMP） by Sugiyama et al. （Anal. Biochem. 1990） and for guanosine 3’,5’-cyclic monophosphate （cGMP） by Seya\net al. （Anal. Biochem. 1998）. However, to make a new simultaneous comparison of two cyclic nucleotides, distinct\nmeasurement methods cause less reliability and longer measurement time. To overcome these problems, we\ndeveloped a simultaneous measurement method for them. All adenosine nucleotides and GMP were enzymatically\ndegraded using alkaline phosphatase and apyrase. The remaining GDP was converted to GTP by creatine kinase.\nCyclic GMP and cAMP, absorbed into a Sep-Pak amino propyl cartridge, were eluted to separate from GTP, and\nthen were simultaneously quantified using improved enzymatic fluorometric assay. The detection limits for cAMP\nand cGMP were 1 and 5 fmol, respectively. The total measurement time was about 10 h. Using this method, the\nbasal cAMP and cGMP levels in rat aortic smooth muscle cells were confirmed to 4.1 and 0.042 pmol/mg protein,\nrespectively. An adrenergic agonist, isoproterenol （1 μM） increased cAMP approximately 6 fold, while nitric oxide\ndonor, S-nitroso-N-acetylpenicillamine （100 μM） increased cGMP approximately 230 fold. These results suggest that\nthis simultaneous measurement of cGMP and cAMP can provide a more convenient assessment of them in biological samples.","subitem_description_type":"Abstract"}]},"item_3_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前医学. 68, p.52-61. 2017","subitem_description_type":"Other"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会","subitem_publisher_language":"ja"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"PISSN"}]},"item_3_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_scheme":"Other"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pharmacology"},{"subitem_text_value":"Department of Pharmacology"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pharmacology"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Seya, Kazuhiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Motomura, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imaizumi, Tadaatsu","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Furukawa, Ken-Ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-13"}],"displaytype":"detail","filename":"HirosakiMedJ_68(1)_52.pdf","filesize":[{"value":"276.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_68(1)_52.pdf","url":"https://hirosaki.repo.nii.ac.jp/record/3925/files/HirosakiMedJ_68(1)_52.pdf"},"version_id":"136f8d5e-ea22-4191-bf27-79f9fa30025d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"adenosine 3’,5’-cyclic monophosphate","subitem_subject_scheme":"Other"},{"subitem_subject":"guanosine 3’,5’-cyclic monophosphate","subitem_subject_scheme":"Other"},{"subitem_subject":"Sep-Pak amino propyl cartridge","subitem_subject_scheme":"Other"},{"subitem_subject":"enzymatic cycling","subitem_subject_scheme":"Other"},{"subitem_subject":"aortic smooth muscle cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simultaneous Measurement of Adenosine 3’,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate in Biological Samples","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simultaneous Measurement of Adenosine 3’,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate in Biological Samples","subitem_title_language":"en"}]},"item_type_id":"3","owner":"3","path":["661"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-10-23"},"publish_date":"2017-10-23","publish_status":"0","recid":"3925","relation_version_is_last":true,"title":["Simultaneous Measurement of Adenosine 3’,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate in Biological Samples"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-22T07:15:49.670225+00:00"}