{"created":"2023-05-15T09:03:14.308563+00:00","id":4122,"links":{},"metadata":{"_buckets":{"deposit":"874553e3-d542-4a29-989f-99cfbc5dd980"},"_deposit":{"created_by":3,"id":"4122","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4122"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00004122","sets":["557:666:669"]},"author_link":["15701","15703","12114","15718","10708","15704","15524","11842","15715","15723","10707","14200","15706","15725","15525","15720","15009","11844","12001","15713","15707","15716","11997","15093","12108"],"item_7_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"704","bibliographicPageStart":"698","bibliographicVolumeNumber":"436","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications"}]}]},"item_7_date_granted_66":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2013-09-30"}]},"item_7_degree_grantor_64":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"弘前大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"11101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_63":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_7_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mesenchymal stem cells (MSCs) have been isolated from various tissues and used for elucidating the pathogenesis of numerous diseases. In our previous in vitro study, we showed the existence of MSCs in human spinal ligaments and hypothesized that these MSCs contributed to the pathogenesis of ossification of spinal ligaments. The purpose of this study was to use immunohistochemical techniques to analyze the localization of MSCs in ossified human spinal ligaments in situ. Ossified (OLF) or non-ossified ligamentum flavum (non-OLF) samples from the thoracic vertebra were obtained from patients who had undergone posterior spinal surgery. Serial sections were prepared from paraffin-embedded samples, and double immunofluorescence staining was performed using antibodies against markers for MSCs (CD73, CD90 and CD105), endothelial cells (CD31), pericytes (α-smooth muscle actin), and chondrocytes (S100). Immunolocalization of MSCs was observed in the perivascular area and collagenous matrix in spinal ligaments. Markers for MSCs and pericytes were co-expressed in the perivascular area. Compared with non-OLF, OLF had a large amount of neovascularization in the fragmented ligament matrix, and a high accumulation of MSCs around blood vessels. The prevalence of MSCs in OLF within collagenous matrix was significantly higher than that in non-OLF. Chondrocytes near the ossification front in OLF also presented expression of MSC markers. MSCs may contribute to the ectopic ossification process of OLF through endochondral ossification.","subitem_description_type":"Abstract"}]},"item_7_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and biophysical research communications, 436(4), 2013, p.698-704","subitem_description_type":"Other"}]},"item_7_dissertation_number_67":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲1811"}]},"item_7_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"15713","nameIdentifierScheme":"WEKO"}],"names":[{"name":"チン, シュンフ"}]},{"nameIdentifiers":[{"nameIdentifier":"12001","nameIdentifierScheme":"WEKO"}],"names":[{"name":"フルカワ, ケンイチ"}]},{"nameIdentifiers":[{"nameIdentifier":"15715","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オノ, アツシ"}]},{"nameIdentifiers":[{"nameIdentifier":"15716","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アサリ, トオル"}]},{"nameIdentifiers":[{"nameIdentifier":"15525","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ハラダ, ヨシフミ"}]},{"nameIdentifiers":[{"nameIdentifier":"15718","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ワダ, ケンイチロウ"}]},{"nameIdentifiers":[{"nameIdentifier":"15093","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タナカ, トシヒロ"}]},{"nameIdentifiers":[{"nameIdentifier":"15720","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イナバ, ワタル"}]},{"nameIdentifiers":[{"nameIdentifier":"14200","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ミズカミ, ヒロキ"}]},{"nameIdentifiers":[{"nameIdentifier":"12114","nameIdentifierScheme":"WEKO"}],"names":[{"name":"モトムラ, シゲル"}]},{"nameIdentifiers":[{"nameIdentifier":"15723","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヤギハシ, ソウロク"}]},{"nameIdentifiers":[{"nameIdentifier":"10707","nameIdentifierScheme":"WEKO"}],"names":[{"name":"イシバシ, ヤスユキ"}]}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"15725","nameIdentifierScheme":"WEKO"}],"names":[{"name":"陳, 俊輔"}]}]},"item_7_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_7_radio_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_radio_item":"ETD"}]},"item_7_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Thesis"}]},"item_7_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2013.06.019","subitem_relation_type_select":"DOI"}}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_7_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"494.66","subitem_subject_scheme":"NDC"}]},"item_7_subject_23":{"attribute_name":"NIIサブジェクト","attribute_value_mlt":[{"subitem_subject":"外科系臨床医学","subitem_subject_scheme":"Other"}]},"item_7_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chin, Shunfu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furukawa, Ken-Ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asari, Toru"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Harada, Yoshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Kanichiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tanaka, Toshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inaba, 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