{"created":"2023-05-15T09:03:30.985218+00:00","id":4441,"links":{},"metadata":{"_buckets":{"deposit":"b4b51402-18a3-4826-861b-ece29891034b"},"_deposit":{"created_by":3,"id":"4441","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4441"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00004441","sets":["701:705:710"]},"author_link":["16925","16934","16713","16772","16774","16935","16770","16932","16712","16714","16929","16927","16922","16924","16930"],"item_5_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Somaclonal Variation and Comparison to Mutation Induced by X-rays in Rice（Oryza sativa L.）by Using Rice Landmarker"}]},"item_5_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ソマクローナル ヘンイ ト Xセン ショウシャ ニヨル トツゼンヘンイノイネランドマーカーニヨルカイセキトヒカク"}]},"item_5_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"23","bibliographicPageStart":"17","bibliographic_titles":[{"bibliographic_title":"弘前大学農学生命科学部学術報告"}]}]},"item_5_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"この研究では，イネゲノム解読に用いられたランドマーカーセット1，セット2 及びニューセットのDNA クローンを用いてイネ品種「つがるおとめ」及び「むつほまれ」の種子にX 線照射して得られた個体，及び培養細胞からの再分化個体の突然変異をサザンブロット解析で調査した。　セット1，セット2 及びニューセットは合わせて105 のゲノミックDNA クローンと235 のcDNA クローンで構成されている。X 線照射で変異を生じた個体は各品種とも5 個体で同数であったが，特定個体で多くのプローブで多型が認められ，DNA 修復系の異常が推定された。また，レトロトランスポゾンTos17 の転移やメチレーションパターンの変化は認められなかった。一方，培養細胞からの再生個体ではTos17 の転移が見られ，転移位置にホットスポットが存在した。また，メチレーションパターンの変化や反復配列の増大なども報告図4. DNA メチレ－ションパターンの解析。レーン1-6 : Hpa II（メチレイションに感受性）で処理。レーン7-12 : Msp I（メチレイションに非感受性）で処理。プローブはC970 クローンを使用した。ソマクローナル変異とX 線による突然変異のイネにおける比較されており，ソマクローナル変異が，エピジェネテックなメチル化は別としても，イネ育種にとって応用の可能性のあることが示唆された。","subitem_description_type":"Abstract"}]},"item_5_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"弘前大学農学生命科学部学術報告. 8, 2005, p.17-23","subitem_description_type":"Other"}]},"item_5_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"16927","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヤマダ, マコト"}]},{"nameIdentifiers":[{"nameIdentifier":"16772","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ロ, チュウオン"}]},{"nameIdentifiers":[{"nameIdentifier":"16929","nameIdentifierScheme":"WEKO"}],"names":[{"name":"クラタ, ユウスケ"}]},{"nameIdentifiers":[{"nameIdentifier":"16930","nameIdentifierScheme":"WEKO"}],"names":[{"name":"マツナガ, 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