{"created":"2023-05-15T09:04:23.895803+00:00","id":5474,"links":{},"metadata":{"_buckets":{"deposit":"fa9501f7-553a-4b6b-8df5-ea0469012536"},"_deposit":{"created_by":11,"id":"5474","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"5474"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00005474","sets":["953:954:955"]},"author_link":["13944","13943","19754","16061","19758","13751","13752","19755","19757","13980","11855","19759","11610","19760","19756","12239","13749","11890"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"748","bibliographicPageStart":"739","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"CANCER MEDICINE"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Serum biomarker monitoring is essential for management of germ-cell tumors (GCT). However, not all GCT are positive for conventional tumor markers. We examined whether serum N-glycan-based biomarkers can be applied for detection and prognosis in patients with GCT. We performed a comprehensive N-glycan structural analysis of sera from 54 untreated GCT patients and 103 age-adjusted healthy volunteers using glycoblotting methods and mass spectrometry. Candidate N-glycans were selected from those with the highest association; cutoff concentration values were established, and an N-glycan score was created based on the number of positive N-glycans present. The validity of this score for diagnosis and prognosis was analyzed using a receiver operating characteristic (ROC) curve. We identified five candidate N-glycans significantly associated with GCT patients. The accuracy of the N-glycan score for GCT was significant with an area-under-the-curve (AUC) value of 0.87. Diagnostically, the N-glycan score detected 10 of 12 (83%) patients with negative conventional tumor markers. Prognostically, the N-glycan score comprised four candidate N-glycans. The predictive value of the prognostic N-glycan score was significant, with an AUC value of 0.89. A high value prognostic N-glycan score was significantly associated with poor prognosis. Finally, to identify a potential carrier protein, immunoglobulin (Ig) fractions of sera were subjected to N-glycan analysis and compared to whole sera. Candidate N-glycans in Ig-fractions were significantly decreased; therefore, the carrier protein for candidate N-glycans is likely not an immunoglobulin. In summary, our newly developed N-glycan score seems to be a practical diagnostic and prognostic method for GCT.","subitem_description_type":"Abstract"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/cam4.1035","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-7634","subitem_source_identifier_type":"ISSN"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Adv Transplant & Regenerat Med"},{"subitem_text_value":"Akita Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Tohoku Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Tohoku Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Yamagata Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Miyagi Canc Ctr, Dept Urol"},{"subitem_text_value":"Miyagi Canc Ctr, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Sch Med, Dept Social Med"},{"subitem_text_value":"Hirosaki Univ, Sch Med, Dept Social Med"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Yamagata Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Akita Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Tohoku Univ, Grad Sch Med, Dept Urol"},{"subitem_text_value":"Hirosaki Univ, Grad Sch Med, Dept Urol"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Narita, Takuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hatakeyama, Shingo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yoneyama, Tohru"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Narita, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Shinichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitsuzuka, Koji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Toshihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawamura, Sadafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tochigi, Tatsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Ippei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakaji, Shigeyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tobisawa, Yuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yamamoto, Hayato"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Koie, Yoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Norihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Habuchi, Tomonori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arai, Yoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Chikara"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-08"}],"displaytype":"detail","filename":"cam4.1035.pdf","filesize":[{"value":"425.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"cam4.1035","url":"https://hirosaki.repo.nii.ac.jp/record/5474/files/cam4.1035.pdf"},"version_id":"cabf70fa-04f6-4f5a-8cb5-2329b394a03f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Clinical implications of serum N-glycan profiling as a diagnostic and prognostic biomarker in germ-cell tumors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Clinical implications of serum N-glycan profiling as a diagnostic and prognostic biomarker in germ-cell tumors"}]},"item_type_id":"3","owner":"11","path":["955"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-08"},"publish_date":"2019-03-08","publish_status":"0","recid":"5474","relation_version_is_last":true,"title":["Clinical implications of serum N-glycan profiling as a diagnostic and prognostic biomarker in germ-cell tumors"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T11:21:22.232048+00:00"}