{"created":"2023-05-15T09:04:26.035669+00:00","id":5507,"links":{},"metadata":{"_buckets":{"deposit":"c879fa51-9737-4e43-9c2e-086333ae043f"},"_deposit":{"created_by":11,"id":"5507","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"5507"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00005507","sets":["557:561:958"]},"author_link":["19800","19799","19745"],"item_3_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Biochemical Analyses of Molecular Mechanisms of Regulation of Genome functions using the Iocus-Specific Regulation of Genome Functions using the Locus-Specific Chromatin Immunoprecipitation Technologies"}]},"item_3_alternative_title_22":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"イデンシザ　トクイテキ　クロマチン　メンエキ　チンコウ　ホウ　ニ　ヨル　ゲノム　キノウ　ハツゲン　チョウセツ　キコウ　ノ　セイカガク　テキ　カイセキ"}]},"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"18","bibliographicPageStart":"10","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"転写やエピジェネティック制御をはじめとするゲノム機能発現調節の分子機構の解明には，解析対象とするゲノム領域に結合している分子を同定することが必須である．筆者らのグループは，これを実現するための新規方法として「遺伝子座特異的クロマチン免疫沈降法（遺伝子座特異的chromatin immunoprecipitation（ ChIP） 法）」を開発してきた.　遺伝子座特異的ChIP 法は，細胞から分子間相互作用を保持したまま解析対象ゲノム領域を特異的に単離する生化学的技術であり，質量分析法 （mass spectrometry: MS） や次世代シークエンス法 （next-generation sequencing: NGS） などの手法と組み合わせることにより，当該ゲノム領域結合分子の網羅的同定が可能である．遺伝子座特異的ChIP 法は，insertional ChIP（ iChIP） 法とengineered DNA-binding molecule-mediated ChIP（enChIP）法から構成される．本稿では，遺伝子座特異的ChIP 法の基本原理や応用例について紹介する．\nFor comprehensive understanding of molecular mechanisms of regulation of genome functions including transcription and epigenetic regulation, it is essential to identify molecules associated with the genomic regions to be analyzed in vivo. To this end, the author's research group has developed the locus-specific chromatin immunoprecipitation （locus-specific ChIP） technologies consisting of insertional ChIP（ iChIP） and engineered DNA-binding molecule-mediated ChIP（ enChIP）. The locus-specific ChIP technologies are biochemical methods to purify specific genomic regions retaining molecular interactions from cells and combined with mass spectrometry（ MS）, next generation sequencing （NGS）, and other methods to identify molecules associated with the genomic regions. In this review, I describe the basic principles and applications of the locus-specific ChIP technologies.","subitem_description_type":"Abstract"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"19800","nameIdentifierScheme":"WEKO"}],"names":[{"name":"フジイ, ホダカ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"19745","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fujii, Hodaka"}]}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"弘前大学大学院医学研究科ゲノム生化学講座"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤井, 穂高"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-09"}],"displaytype":"detail","filename":"HirosakiMedJ_69(1-4)_10.pdf","filesize":[{"value":"330.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_69(1-4)_10","url":"https://hirosaki.repo.nii.ac.jp/record/5507/files/HirosakiMedJ_69(1-4)_10.pdf"},"version_id":"aca022e3-5702-4eb9-ba8c-7000a95dd66d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クロマチン免疫沈降法","subitem_subject_scheme":"Other"},{"subitem_subject":"ChIP","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子座特異的ChIP法","subitem_subject_scheme":"Other"},{"subitem_subject":"iChIP","subitem_subject_scheme":"Other"},{"subitem_subject":"enChIP.","subitem_subject_scheme":"Other"},{"subitem_subject":"ChIP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chromatin immunoprecipitation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"locus-specific ChIP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"iChIP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enChIP","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"遺伝子座特異的クロマチン免疫沈降法によるゲノム機能発現調節機構の生化学的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子座特異的クロマチン免疫沈降法によるゲノム機能発現調節機構の生化学的解析"}]},"item_type_id":"3","owner":"11","path":["958"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-09"},"publish_date":"2019-04-09","publish_status":"0","recid":"5507","relation_version_is_last":true,"title":["遺伝子座特異的クロマチン免疫沈降法によるゲノム機能発現調節機構の生化学的解析"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-16T06:19:56.780219+00:00"}