{"created":"2023-05-15T09:04:26.553366+00:00","id":5515,"links":{},"metadata":{"_buckets":{"deposit":"f1ad91f6-7b09-41ef-ad0c-767d7ede47ca"},"_deposit":{"created_by":11,"id":"5515","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"5515"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00005515","sets":["557:561:958"]},"author_link":["14291","12410","19490","13908","11459","19841","11302","11844","11697","11458","14716","19838"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"94","bibliographicPageStart":"86","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Retinoic acid-inducible gene-I （RIG-I） is a member of cytoplasmic viral sensors which plays an important role in inflammation of biliary epithelial cells（ BECs）. The aim of this study is to examine if RIG-I and C-X-C motif chemokine 10（ CXCL10） are involved in the etiology of human biliary atresia（ BA）.\nMethods: Immunohistochemical study was performed on surgically resected tissues obtained（ June 1994 to March 2016）from 30 infants with BA and non-inflamed hepatic tissues from 7 infants with hepatoblastoma. A semiquantitative scoring system was designed to evaluate the staining with an antibodies to the RIG-I and CXCL10. The expression of RIG-I and CXCL10 in HuCCT1 cholangiocarcinoma cell line were studied by western blotting, ELISA and RT-PCR analyses.\nResults: Intense immunoreactivity for RIG-I and CXCL10 was detected in BECs in tissues resected from BA patients.The expression of RIG-I and CXCL10 in the hilar tissue was significantly stronger than in the hepatic tissue.Transfection of HuCCT1 cells with poly（I:C）, a synthetic analog of viral dsRNA, induced the expression of RIG-I, and knockdown of RIG-I inhibited the induction of CXCL10 in HuCCT1 cells transfected with poly（I:C）.\nConclusion: These results suggest that RIG-I-CXCL10 cascade may be involved in the etiology of human BA.","subitem_description_type":"Abstract"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_19":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Gastroenterology and Hematology,Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital"},{"subitem_text_value":"Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital"},{"subitem_text_value":"Department of Pathology and Molecular Medicine,Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pediatric Surgery, Hirosaki University School of Medicine and Hospital"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kimura, Toshiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imaizumi, Tadaatsu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yoshida, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimada, Taku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayakari, Ryo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawaguchi, Shogo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yoshida, Hidemi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Tamotsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirabayashi, Takeshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Mizukami, Hiroki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kijima, Hiroshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hakamada, Kenichi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-09"}],"displaytype":"detail","filename":"HirosakiMedJ_69(1-4)_86.pdf","filesize":[{"value":"489.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_69(1-4)_86.pdf ","url":"https://hirosaki.repo.nii.ac.jp/record/5515/files/HirosakiMedJ_69(1-4)_86.pdf"},"version_id":"6d1fc61d-59c3-47bb-9ad2-eb18738c9ab1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"biliary atresia","subitem_subject_scheme":"Other"},{"subitem_subject":"retinoic acid-inducible gene-I","subitem_subject_scheme":"Other"},{"subitem_subject":"CXCL10","subitem_subject_scheme":"Other"},{"subitem_subject":"HuCCT1","subitem_subject_scheme":"Other"},{"subitem_subject":"poly（I:C）.","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia"}]},"item_type_id":"3","owner":"11","path":["958"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-09"},"publish_date":"2019-04-09","publish_status":"0","recid":"5515","relation_version_is_last":true,"title":["Retinoic Acid-Inducible Gene-i and CXCL10 are Involved in Biliary Atresia"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T10:40:27.454757+00:00"}