{"created":"2023-05-15T09:04:39.251939+00:00","id":5777,"links":{},"metadata":{"_buckets":{"deposit":"ab4746b8-6ab7-4090-be89-08f6ced8d6ef"},"_deposit":{"created_by":11,"id":"5777","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"5777"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00005777","sets":["557:561:976"]},"author_link":["11238","12262","10687","20292"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"弘前医学"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract: Hyaluronan （HA） is a ubiquitous glycosaminoglycan comprising N-acetylglucosamine and glucuronic acid and is involved in matrix formation and cell maintenance. 4-Methylumbelliferone （MU） exhibits anti-tumor activities by inhibiting HA synthesis. In this study, we aimed to elucidate the association between HA and numerous matrix molecules.\nMaterials and Methods: We created HA-knock down extracellular matrix （ECM） by culturing human skin fibroblasts（ HSF） in the presence of MU（ 0.5 mM） for 3 days after confluence. Following culture in the presence or absence of MU, the cell layer and culture medium were collected and ECM molecules were measured using enzymelinked immunosolvent assay, and the［ 35S］-sulfate uptake method.\nResults: Proteoglycans and type I collagen were released into the medium as HA decreased. However, type collagen, but not proteoglycans, was immediately replenished. Fibronectin and CD44 in the cell layer decreased, but were not replenished, as HA decreased. On contrary, laminin, cadherin, and vitronectin were not affected by HA decrease.\nConclusions: HA knockdown by MU treatment elucidated the dynamic interactions between HA and ECM components. Our findings suggest that HA interacts with other ECM components through various ways, including","subitem_description_type":"Abstract"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科・弘前医学会"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Anatomical Science, Hirosaki University Graduate School of Health Sciences"},{"subitem_text_value":"Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Endo, Yasufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Gen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kakizaki, Ikuko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Endo, Masahiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-11-22"}],"displaytype":"detail","filename":"HirosakiMedJ_70(1)_1.pdf","filesize":[{"value":"315.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HirosakiMedJ_70(1)_1","url":"https://hirosaki.repo.nii.ac.jp/record/5777/files/HirosakiMedJ_70(1)_1.pdf"},"version_id":"48f1412e-e898-4642-913c-fe7b574067b6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hyaluronan","subitem_subject_scheme":"Other"},{"subitem_subject":"hyaluronan synthesis inhibitor","subitem_subject_scheme":"Other"},{"subitem_subject":"4-methylumbelliferone","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular matrix","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts"}]},"item_type_id":"3","owner":"11","path":["976"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-22"},"publish_date":"2019-11-22","publish_status":"0","recid":"5777","relation_version_is_last":true,"title":["Hyaluronan synthesis inhibitor 4-methylumbelliferone affects extracellular matrix components in cultured human skin fibroblasts"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T10:19:32.315875+00:00"}