@article{oai:hirosaki.repo.nii.ac.jp:00006186,
 author = {Ogasawara, shu and Saito, Norihiro and Minakawa, Satoko and Akasaka, Tomomi and Kayaba, Hiroyuki},
 issue = {2-4},
 journal = {弘前医学},
 month = {Mar},
 note = {Background: The Duffy antigen receptor for chemokines （DARC） is expressed on RBCs and is a non-signalingreceptor for multiple chemokines. In the previous study, we had shown that DARC-affinity chemokines are stored inthe RBCs. However, the fate of intracellularly stored chemokines has yet to be investigated. This study investigatedwhether RBCs retain the ability to accumulate these chemokines as they age.
Methods: Peripheral blood from five healthy volunteers were collected, and then transferred to hematocrit capillarytubes. After centrifugation, the RBC layer was divided to compare the top, upper, middle and bottom fractions.HbA1c, eotaxin-1 and RANTES were measured in each fraction.
Results: HbA1c increased significantly from the top fraction to the bottom fraction. Eotaxin-1 decreased significantlyfrom the top fraction to the bottom fraction. RANTES exhibited a similar decrease, although no significant differenceswere observed. Eotaxin-1 showed a negative correlation（ r = -0.51, p = 0.03） with HbA1c. Although not statisticallysignificant, RANTES showed a decreasing tendency （r = -0.40, p = 0.08） with the increase of HbA1c levels. Theseresults indicate that RANTES and eotaxin-1 decrease rather than increase with RBC aging.
Conclusion: Eotaxin-1 and RANTES stored in RBCs tended to decrease as RBCs age.},
 pages = {113--119},
 title = {Relationship between red blood cell aging and intracellular chemokine storage},
 volume = {71},
 year = {2021}
}