{"created":"2023-05-15T09:05:48.430812+00:00","id":7176,"links":{},"metadata":{"_buckets":{"deposit":"f35f75e5-69c4-407d-873b-a784efed9ada"},"_deposit":{"created_by":11,"id":"7176","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"7176"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00007176","sets":["557:561:1115"]},"author_link":["14831","11403","11997","11879","19746","11458","2405"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"29","bibliographicPageStart":"20","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"弘前医学"},{"bibliographic_title":"Hirosaki Medical Journal","bibliographic_titleLang":"en"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Wingless/integrase 5a（Wnt5a） pathway is known to regulate the osteogenesis. In this study, we aimed to clarify the role of Wnt5a pathway in aortic valve ectopic calcification.\nMethods: Human aortic valve interstitial cells（HAVICs） were obtained from calcified aortic valves of patients with calcific aortic valve stenosis（CAVS）. HAVICs were separated to CD34-negative and -positive cells by flow cytometry. We measured real-time PCR, alkaline phosphatase（ALP） activity, Alizarin Red S staining as an index of calcification. Immunohistochemical staining was performed to confirm the distribution of Wnt5a on calcified and normal aortic valves.\nResults: HAVICs, especially, CD34-negative cells are highly sensitive to tumor necrosis factor-α（ TNF-α, 30 ng/mL）, and which accelerated the Wnt5a gene expression. Wnt5a antagonist, box5, did not down-regulate HAVIC calcification induced by TNF-α. Further, Wnt5a agonist, foxy5, did not accelerated HAVIC calcification induced by TNF-α. There was no significant difference in the TNF-α-induced acceleration of ALP activity between the cells treated with foxy5 and box5. Furthermore, the proportion of Wnt5a positive cells was no difference between calcified and normal valves.\nConclusions: We confirmed that Wnt5a pathway does not regulate the TNF-α-induced aortic valve calcification in HAVICs obtained from CAVS patients.","subitem_description_type":"Abstract"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Thoracic and Cardiovascular Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Pharmacology, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yu, Zaiqiang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Daitoku, Kazuyuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Fukuda, Ikuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imaizumi, Tadaatsu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Minakawa, Masahito"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Furukawa, Ken-Ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seya, Kazuhiko"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-21"}],"displaytype":"detail","filename":"Hirosaki Med J 73-1-4_20.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Hirosaki Med J 73-1-4_20","url":"https://hirosaki.repo.nii.ac.jp/record/7176/files/Hirosaki Med J 73-1-4_20.pdf"},"version_id":"aa455e32-688e-4fb1-b7a3-b6b4df61fa0b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Calcific aortic valve stenosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Aortic valve calcification","subitem_subject_scheme":"Other"},{"subitem_subject":"Wingless/integrase 5A","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone morphogenetic protein 2","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis"}]},"item_type_id":"3","owner":"11","path":["1115"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-04-21"},"publish_date":"2023-04-21","publish_status":"0","recid":"7176","relation_version_is_last":true,"title":["Wnt5a pathway was not involved in the progression of valve calcification in calcific aortic valve stenosis"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T09:28:13.186523+00:00"}