{"created":"2023-05-15T09:05:48.497094+00:00","id":7177,"links":{},"metadata":{"_buckets":{"deposit":"2e1d7ecb-8991-4ac3-bc63-90b57b21f410"},"_deposit":{"created_by":11,"id":"7177","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"7177"},"status":"published"},"_oai":{"id":"oai:hirosaki.repo.nii.ac.jp:00007177","sets":["557:561:1115"]},"author_link":["22477","14294","20947","22476","22475","11871","14292","11458","11868"],"item_3_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-4","bibliographicPageEnd":"39","bibliographicPageStart":"30","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"弘前医学"},{"bibliographic_title":"Hirosaki Medical Journal","bibliographic_titleLang":"en"}]}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Medication-related osteonecrosis of the jaw （MRONJ） is a complication of bisphosphonate （BP） therapy. The pathophysiology of MRONJ remains unclear. Extensive studies have been performed to examine the effect of BPs on bone metabolism. Breakdown of mucosa is a factor classified into five stages in MRONJ, indicating an important role of oral soft tissue in the pathogenesis of MRONJ.\nMethods: We investigated the effect of alendronate, one of the most commonly used BPs worldwide, on the proliferation of human gingival fibroblasts by MTT assay, western blotting, and wound healing assay.\nResults: We observed time- and concentration-dependent inhibition of fibroblast proliferation by alendronate. Wound healing assays also showed that alendronate prolonged wound healing in a concentration-dependent manner. MAP kinase signaling pathway exhibits a cardinal role in cell proliferation; however, little is known about whether BPs affect classical MAP kinase ERK in fibroblasts. We found that U0126, a selective inhibitor of MAP kinase kinase （MEK1/2）, inhibited fibroblast proliferation similarly to alendronate. Alendronate was also found to inactivate ERK1/2, both of which are downstream molecules of MEK1/2.\nConclusion: our findings indicated that alendronate inhibits the proliferation of human gingival fibroblasts via inactivation of ERK1/2.","subitem_description_type":"Abstract"}]},"item_3_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前大学大学院医学研究科"}]},"item_3_radio_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_radio_item":"Article"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00211444","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0439-1721","subitem_source_identifier_type":"ISSN"}]},"item_3_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine"},{"subitem_text_value":"Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine / Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences"},{"subitem_text_value":"Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine"}]},"item_3_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Akiyama, Natsumi"}],"nameIdentifiers":[{"nameIdentifier":"20947","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Kosei"}],"nameIdentifiers":[{"nameIdentifier":"11868","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"100000220","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/100000220_ja.html"}]},{"creatorNames":[{"creatorName":"Komatsu, Shotaro"}],"nameIdentifiers":[{"nameIdentifier":"22475","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Ryohei"}],"nameIdentifiers":[{"nameIdentifier":"14292","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chang, Hao Chi"}],"nameIdentifiers":[{"nameIdentifier":"22476","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamazaki, Shunya"}],"nameIdentifiers":[{"nameIdentifier":"22477","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imaizumi, Tadaatsu"}],"nameIdentifiers":[{"nameIdentifier":"11458","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"318","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/318_ja.html"}]},{"creatorNames":[{"creatorName":"Matsumiya, Tomoh"}],"nameIdentifiers":[{"nameIdentifier":"11871","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"319","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/319_ja.html"}]},{"creatorNames":[{"creatorName":"Kobayashi, Wataru"}],"nameIdentifiers":[{"nameIdentifier":"14294","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"309","nameIdentifierScheme":"研究者総覧","nameIdentifierURI":"http://hue2.jm.hirosaki-u.ac.jp/html/309_ja.html"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-21"}],"displaytype":"detail","filename":"Hirosaki Med J 73-1-4_30.pdf","filesize":[{"value":"413.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Hirosaki Med J 73-1-4_30","url":"https://hirosaki.repo.nii.ac.jp/record/7177/files/Hirosaki Med J 73-1-4_30.pdf"},"version_id":"ee71aad6-2767-4ac4-a23c-7c9ba55ce974"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MRONJ","subitem_subject_scheme":"Other"},{"subitem_subject":"alendronate","subitem_subject_scheme":"Other"},{"subitem_subject":"Bisphosphonate","subitem_subject_scheme":"Other"},{"subitem_subject":"gingival fibroblasts","subitem_subject_scheme":"Other"},{"subitem_subject":"MAP kinas","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of bisphosphonate alendronate on ERK-dependent growth in human gingival fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of bisphosphonate alendronate on ERK-dependent growth in human gingival fibroblasts"}]},"item_type_id":"3","owner":"11","path":["1115"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-04-21"},"publish_date":"2023-04-21","publish_status":"0","recid":"7177","relation_version_is_last":true,"title":["Effect of bisphosphonate alendronate on ERK-dependent growth in human gingival fibroblasts"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T09:28:09.747881+00:00"}